Domain organization of flagellar hook protein from Salmonella typhimurium

Hatsuho Uedaira, Hisayuki Morii, Miyuki Ishimura, Hisaaki Taniguchi, Keiichi Namba, F. Vonderviszt

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hook forms a universal joint, which mediates the torque of the flagellar motor to the outer helical filaments. Domain organization of hook protein from Salmonella typhimurium was investigated by exploring thermal denaturation properties of its proteolytic fragments. The most stable part of hook protein involves residues 148 to 355 and consists of two domains, as revealed by deconvolution analysis of the calorimetric melting profiles. Residues 72-147 and 356-370 form another domain, while the terminal regions of the molecule, residues 1-71 and 371-403, avoid a compact tertiary structure in the monomeric state. These folding domains were assigned to the morphological domains of hook subunits known from EM image reconstructions, revealing the overall folding of hook protein in its filamentous state. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalFEBS Letters
Volume445
Issue number1
DOIs
Publication statusPublished - Feb 19 1999

Fingerprint

Computer-Assisted Image Processing
Hooks
Protein Folding
Torque
Freezing
Hot Temperature
Joints
Proteins
Universal joints
Denaturation
Deconvolution
Image reconstruction
Melting
Molecules
Bacteria FlgE protein

Keywords

  • Bacterial flagellum
  • Calorimetry
  • Domain structure
  • Hook
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Domain organization of flagellar hook protein from Salmonella typhimurium. / Uedaira, Hatsuho; Morii, Hisayuki; Ishimura, Miyuki; Taniguchi, Hisaaki; Namba, Keiichi; Vonderviszt, F.

In: FEBS Letters, Vol. 445, No. 1, 19.02.1999, p. 126-130.

Research output: Contribution to journalArticle

Uedaira, H, Morii, H, Ishimura, M, Taniguchi, H, Namba, K & Vonderviszt, F 1999, 'Domain organization of flagellar hook protein from Salmonella typhimurium', FEBS Letters, vol. 445, no. 1, pp. 126-130. https://doi.org/10.1016/S0014-5793(99)00110-6
Uedaira, Hatsuho ; Morii, Hisayuki ; Ishimura, Miyuki ; Taniguchi, Hisaaki ; Namba, Keiichi ; Vonderviszt, F. / Domain organization of flagellar hook protein from Salmonella typhimurium. In: FEBS Letters. 1999 ; Vol. 445, No. 1. pp. 126-130.
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