DNA synthesis and nucleoside metabolism in human tonsillar lymphocyte subpopulations

M. Staub, T. Spasokukotskaja, M. Benczur, F. Antoni

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

High (HD) and low (LD) density cells were separated on 25% BSA gradient from tonsils of 3-6 years old children. Early B lymphocyte markers and slg-s were found on the surface of 59-82% of the LD cells. This cell population was 5-6 times more active in DNA synthesis (3H-thymidine incorporation, DNA polymerase activity) than the HD cells. The total uptake of 3H-deoxycytidine was about the same as that of 3H-thymidine. As long as practically all thymidine taken up by the cells was immediately incorporated into DNA (90-95% only 10-15% of deoxycytidine was incorporated into DNA under the same conditions, indicating different pool sizes for the DNA precursors. The majority of deoxycytidine (70% was converted and incorporated as dTMP. A considerable part of labeled deoxycytidine could be detected in the soluble pool in form of nucleotides (3-8% and in an unknown form, called substance X (8-14% Substance X was purified by TL chromatography and identified by HPLC as deoxycytidine containing liponucleotides, probable precursors for plasmamembranes. The preferential utilisation of deoxycytidine for DNA and membrane synthesis in immature B lymphocytes draws the attention to its function in early events of lymphocyte maturation.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalActa Oto-Laryngologica
Volume105
Issue numberS454
DOIs
Publication statusPublished - 1988

Fingerprint

Deoxycytidine
Lymphocyte Subsets
Nucleosides
DNA
Thymidine
B-Lymphoid Precursor Cells
Palatine Tonsil
DNA-Directed DNA Polymerase
Chromatography
B-Lymphocytes
Nucleotides
Cell Count
High Pressure Liquid Chromatography
Lymphocytes
Membranes
Population

Keywords

  • Deoxynucleoside
  • Differentiation
  • Tonsil

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

DNA synthesis and nucleoside metabolism in human tonsillar lymphocyte subpopulations. / Staub, M.; Spasokukotskaja, T.; Benczur, M.; Antoni, F.

In: Acta Oto-Laryngologica, Vol. 105, No. S454, 1988, p. 118-124.

Research output: Contribution to journalArticle

Staub, M. ; Spasokukotskaja, T. ; Benczur, M. ; Antoni, F. / DNA synthesis and nucleoside metabolism in human tonsillar lymphocyte subpopulations. In: Acta Oto-Laryngologica. 1988 ; Vol. 105, No. S454. pp. 118-124.
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