DNA synthesis and nucleoside metabolism in human tonsillar lymphocyte subpopulations

M. Staub, T. Spasokukotskaja, M. Benczur, F. Antoni

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

High (HD) and low (LD) density cells were separated on 25% BSA gradient from tonsils of 3-6 years old children. Early B lymphocyte markers and slg-s were found on the surface of 59-82% of the LD cells. This cell population was 5-6 times more active in DNA synthesis (3H-thymidine incorporation, DNA polymerase activity) than the HD cells. The total uptake of 3H-deoxycytidine was about the same as that of 3H-thymidine. As long as practically all thymidine taken up by the cells was immediately incorporated into DNA (90-95% only 10-15% of deoxycytidine was incorporated into DNA under the same conditions, indicating different pool sizes for the DNA precursors. The majority of deoxycytidine (70% was converted and incorporated as dTMP. A considerable part of labeled deoxycytidine could be detected in the soluble pool in form of nucleotides (3-8% and in an unknown form, called substance X (8-14% Substance X was purified by TL chromatography and identified by HPLC as deoxycytidine containing liponucleotides, probable precursors for plasmamembranes. The preferential utilisation of deoxycytidine for DNA and membrane synthesis in immature B lymphocytes draws the attention to its function in early events of lymphocyte maturation.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalActa Oto-Laryngologica
Volume105
Issue numberS454
DOIs
Publication statusPublished - Jan 1 1988

    Fingerprint

Keywords

  • Deoxynucleoside
  • Differentiation
  • Tonsil

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this