DNA sequencing by CE

Barry L. Karger, A. Guttman

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Sequencing of human and other genomes has been at the center of interest in the biomedical field over the past several decades and is now leading toward an era of personalized medicine. During this time, DNA-sequencing methods have evolved from the labor-intensive slab gel electrophoresis, through automated multiCE systems using fluorophore labeling with multispectral imaging, to the "next-generation" technologies of cyclic-array, hybridization based, nanopore and single molecule sequencing. Deciphering the genetic blueprint and follow-up confirmatory sequencing of Homo sapiens and other genomes were only possible with the advent of modern sequencing technologies that were a result of step-by-step advances with a contribution of academics, medical personnel and instrument companies. While next-generation sequencing is moving ahead at breakneck speed, the multicapillary electrophoretic systems played an essential role in the sequencing of the Human Genome, the foundation of the field of genomics. In this prospective, we wish to overview the role of CE in DNA sequencing based in part of several of our articles in this journal.

Original languageEnglish
JournalElectrophoresis
Volume30
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Human Genome
DNA Sequence Analysis
Genes
Nanopores
Technology
Precision Medicine
DNA
Genomics
Personnel
Electrophoresis
Blueprints
Fluorophores
Gels
Genome
Labeling
Medicine
Imaging techniques
Molecules
Industry

Keywords

  • CE
  • DNA
  • Sequencing

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

DNA sequencing by CE. / Karger, Barry L.; Guttman, A.

In: Electrophoresis, Vol. 30, No. SUPPL. 1, 06.2009.

Research output: Contribution to journalArticle

Karger, Barry L. ; Guttman, A. / DNA sequencing by CE. In: Electrophoresis. 2009 ; Vol. 30, No. SUPPL. 1.
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