DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals

Takashi Sugita, K. Takeo, K. Hama, E. Virtudazo, M. Takashima, A. Nishikawa, J. Kucsera, J. Dorogi, S. Komori, K. Nakagaki, A. Vollekova, E. Slavikova, V. Farkas

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Abstract

The non-lipid-dependent species Malassezia pachydermatis is frequently isolated from animals. We analyzed the DNA sequences of the intergenic spacer (IGS) 1 region, which is the most variable region in the rRNA gene, of 43 M. pachydermatis strains obtained from dogs or cats. The lengths of the IGS 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these IGS 1 regions were divided into three major groups with 10 subtypes. Group 1 (552-601 bp long) was characterized by the short sequence repeat (CAGCA) n and had four to 14 repeats, and Group 3 (749-898 bp long), which included the neotype strain of M. pachydermatis, was characterized by the sequence (CAGCA-TAACATAACACACAACA) n in the IGS1 region. Group 2 possessed partial sequences of both Groups 1 and 3. Each group shared only 41.7-55.4% similarity in the IGS1 region with the other groups. The internal transcribed spacer (ITS) region and D1/D2 26S rDNA in the rRNA gene were also sequenced for representative strains in each IGS group. The groups were distinguished by both ITS (698-712 bp long including 5.8S rDNA) and D1/D2 26S rDNA (624 bp long) sequences with sequence similarities of 91.7-96.0% and 99.7-99.0%, respectively. Our results indicate that the sequence of the IGS region of M. pachydermatis has a remarkable intraspecies diversity, compared with ITS or D1/D2 26S rDNA, and that multiple genotypic strains of M. pachydermatis colonize animal skin.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalMedical Mycology
Volume43
Issue number1
DOIs
Publication statusPublished - 2005

Fingerprint

Malassezia pachydermatis
Malassezia
Ribosomal DNA
intergenic DNA
nucleotide sequences
internal transcribed spacers
rRNA Genes
animals
Intergenic DNA
ribosomal RNA
neotypes
Cats
skin (animal)
Dogs
genes
Skin
sequence diversity
cats
dogs

Keywords

  • Intergenic spacer (IGS)
  • Malassezia pachydermatis
  • rDNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals. / Sugita, Takashi; Takeo, K.; Hama, K.; Virtudazo, E.; Takashima, M.; Nishikawa, A.; Kucsera, J.; Dorogi, J.; Komori, S.; Nakagaki, K.; Vollekova, A.; Slavikova, E.; Farkas, V.

In: Medical Mycology, Vol. 43, No. 1, 2005, p. 21-26.

Research output: Contribution to journalArticle

Sugita, T, Takeo, K, Hama, K, Virtudazo, E, Takashima, M, Nishikawa, A, Kucsera, J, Dorogi, J, Komori, S, Nakagaki, K, Vollekova, A, Slavikova, E & Farkas, V 2005, 'DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals', Medical Mycology, vol. 43, no. 1, pp. 21-26. https://doi.org/10.1080/1369378042000193185
Sugita, Takashi ; Takeo, K. ; Hama, K. ; Virtudazo, E. ; Takashima, M. ; Nishikawa, A. ; Kucsera, J. ; Dorogi, J. ; Komori, S. ; Nakagaki, K. ; Vollekova, A. ; Slavikova, E. ; Farkas, V. / DNA sequence diversity of intergenic spacer I region in the non-lipid-dependent species Malassezia pachydermatis isolated from animals. In: Medical Mycology. 2005 ; Vol. 43, No. 1. pp. 21-26.
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AU - Virtudazo, E.

AU - Takashima, M.

AU - Nishikawa, A.

AU - Kucsera, J.

AU - Dorogi, J.

AU - Komori, S.

AU - Nakagaki, K.

AU - Vollekova, A.

AU - Slavikova, E.

AU - Farkas, V.

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AB - The non-lipid-dependent species Malassezia pachydermatis is frequently isolated from animals. We analyzed the DNA sequences of the intergenic spacer (IGS) 1 region, which is the most variable region in the rRNA gene, of 43 M. pachydermatis strains obtained from dogs or cats. The lengths of the IGS 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these IGS 1 regions were divided into three major groups with 10 subtypes. Group 1 (552-601 bp long) was characterized by the short sequence repeat (CAGCA) n and had four to 14 repeats, and Group 3 (749-898 bp long), which included the neotype strain of M. pachydermatis, was characterized by the sequence (CAGCA-TAACATAACACACAACA) n in the IGS1 region. Group 2 possessed partial sequences of both Groups 1 and 3. Each group shared only 41.7-55.4% similarity in the IGS1 region with the other groups. The internal transcribed spacer (ITS) region and D1/D2 26S rDNA in the rRNA gene were also sequenced for representative strains in each IGS group. The groups were distinguished by both ITS (698-712 bp long including 5.8S rDNA) and D1/D2 26S rDNA (624 bp long) sequences with sequence similarities of 91.7-96.0% and 99.7-99.0%, respectively. Our results indicate that the sequence of the IGS region of M. pachydermatis has a remarkable intraspecies diversity, compared with ITS or D1/D2 26S rDNA, and that multiple genotypic strains of M. pachydermatis colonize animal skin.

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