DNA profiling by detection of repetitive nucleotide sequences on human chromosome 6

L. Keresztury, A. Lászik, H. Hegyesi, A. Falus

Research output: Contribution to journalArticle

Abstract

Genetic/genomic polymorphism, i.e. variations in DNA sequences are ideally assayed by direct nucleotide sequencing of a gene region or other homologous segment of the genome. An easier and cheaper approach, however, if the variants are analyzed by hybridization technology using restriction fragment length polymorphisms (RFLPs) or by detection of the number of tandem repeats (VNTR) of small DNA segments, the "minisatellites". In this study we describe results of the DNA analysis of repetitive sequences of human 6th chromosome by the application of a chemiluminescent labeled probes. The allele frequency distribution of polymorphic DNA sequences has been determined in unrelated individuals. The isolated genomic DNA was cut with Pst I restriction enzyme, size fractionated on agarose gel and hybridized with a chemiluminescent labeled D6 S132 probe. At this locus the Pst I cleaved DNA fragments are ranging from 1841 to 6098 base pairs (bp). Specific genetic pattern was characterized by more frequent fragments (3313 and 3884 bp), and the rarely occurring ones (clustered between 1841-2595 and 5227-6098 bp). Our study provides a further possibility for characterization of individual genomic patterns.

Original languageEnglish
Pages (from-to)495-498
Number of pages4
JournalActa Biologica Hungarica
Volume53
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Chromosomes, Human, Pair 6
DNA Fingerprinting
Nucleic Acid Repetitive Sequences
Human Chromosomes
Chromosomes
DNA fingerprinting
chromosome
Nucleotides
Base Pairing
chromosomes
nucleotide sequences
DNA
minisatellite repeats
DNA sequences
Polymorphism
genomics
Genes
Minisatellite Repeats
Tandem Repeat Sequences
tandem repeat sequences

Keywords

  • Chemiluminescent probe
  • Forensic medicine
  • Gene technology
  • RFLP
  • VNTR

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

DNA profiling by detection of repetitive nucleotide sequences on human chromosome 6. / Keresztury, L.; Lászik, A.; Hegyesi, H.; Falus, A.

In: Acta Biologica Hungarica, Vol. 53, No. 4, 2002, p. 495-498.

Research output: Contribution to journalArticle

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