DNA profiling by capillary array electrophoresis with non-covalent fluorescent labeling

Nels A. Olson, Julia Khandurina, A. Guttman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Increasing need for large-scale DNA profiling necessitated the development of automated electrophoresis based methods enabling rapid, high performance analysis of nucleic acids in a wide molecular-mass range. In this paper, we report on the adaptation of a commercial 96-capillary array electrophoresis (CAE) instrument for high-throughput DNA fragment analysis and the evaluation of the effects of different non-covalent DNA staining dyes on separation efficiency. The applicability of different color internal fluorescent standards is shown with mathematical spectral overlap correction algorithms. Large-scale quality control assessment of oligonucleotide probes using non-covalent fluorophore labeling is also demonstrated. The method requires small sample amounts, offers automation and quantification capabilities to accommodate modern biotechnology industry needs.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalJournal of Chromatography A
Volume1051
Issue number1-2
DOIs
Publication statusPublished - Oct 8 2004

Fingerprint

DNA Fingerprinting
Capillary Electrophoresis
Electrophoresis
Labeling
Oligonucleotide Probes
Automation
DNA
Biotechnology
Quality Control
Nucleic Acids
Industry
Coloring Agents
Fluorophores
Color
Molecular mass
Staining and Labeling
Quality control
Throughput

Keywords

  • DNA profiling
  • Electrophoresis
  • Non-covalent fluorescent labeling

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

DNA profiling by capillary array electrophoresis with non-covalent fluorescent labeling. / Olson, Nels A.; Khandurina, Julia; Guttman, A.

In: Journal of Chromatography A, Vol. 1051, No. 1-2, 08.10.2004, p. 155-160.

Research output: Contribution to journalArticle

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