DNA polymorphism of MHC III genes in inbred and wild mouse strains

A. Falus, Edward K. Wakeland, Thomas J. McConnell, Jonathan Gitlin, Alexander S. Whitehead, Harvey R. Colten

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Genes encoding the second component (C2), factor B, and complement protein C4 and Slp (sex-limited protein) are members of the major histocompatibility complex class III gene cluster. In this report we describe isolation of a mouse C2 cDNA clone and its use together with factor B and C4 cDNA clones to examine the S region in a panel of 42 haplotypes in laboratory and wild mice representing 5 species and subspecies of Mus. Conservation of the C2 factor B gene duplex was evidenced by relatively limited polymorphism associated with speciation and nucleotide sequence homology between mouse and human C2 and factor B The C4-Slp gene duplex, on the other hand, showed extensive polymorphism by DNA blot analysis. This polymorphism correlated poorly with the C2/factor B restriction fragment length polymorphism, suggesting independent evolution of these two segments of the S region. Taken together, these data will be of particular importance in studies of mouse strains with abnormal regulation of immune effector systems since the class III gene products are essential for activation of the complement cascade.

Original languageEnglish
Pages (from-to)290-298
Number of pages9
JournalImmunogenetics
Volume25
Issue number5
DOIs
Publication statusPublished - May 1987

Fingerprint

Inbred Strains Mice
Complement Factor B
DNA
Genes
Complementary DNA
Clone Cells
Complement C4
Complement Activation
Essential Genes
Multigene Family
Sequence Homology
Major Histocompatibility Complex
Restriction Fragment Length Polymorphisms
Haplotypes
Immune System
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Falus, A., Wakeland, E. K., McConnell, T. J., Gitlin, J., Whitehead, A. S., & Colten, H. R. (1987). DNA polymorphism of MHC III genes in inbred and wild mouse strains. Immunogenetics, 25(5), 290-298. https://doi.org/10.1007/BF00404421

DNA polymorphism of MHC III genes in inbred and wild mouse strains. / Falus, A.; Wakeland, Edward K.; McConnell, Thomas J.; Gitlin, Jonathan; Whitehead, Alexander S.; Colten, Harvey R.

In: Immunogenetics, Vol. 25, No. 5, 05.1987, p. 290-298.

Research output: Contribution to journalArticle

Falus, A, Wakeland, EK, McConnell, TJ, Gitlin, J, Whitehead, AS & Colten, HR 1987, 'DNA polymorphism of MHC III genes in inbred and wild mouse strains', Immunogenetics, vol. 25, no. 5, pp. 290-298. https://doi.org/10.1007/BF00404421
Falus A, Wakeland EK, McConnell TJ, Gitlin J, Whitehead AS, Colten HR. DNA polymorphism of MHC III genes in inbred and wild mouse strains. Immunogenetics. 1987 May;25(5):290-298. https://doi.org/10.1007/BF00404421
Falus, A. ; Wakeland, Edward K. ; McConnell, Thomas J. ; Gitlin, Jonathan ; Whitehead, Alexander S. ; Colten, Harvey R. / DNA polymorphism of MHC III genes in inbred and wild mouse strains. In: Immunogenetics. 1987 ; Vol. 25, No. 5. pp. 290-298.
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