The authors present three DNA fingerprinting methods mostly used in bacterial strain typing. Application of the highly discriminative pulsed-field gel electrophoresis (PFGE), the repetitive element specific PCR (rep-PCR) and the random amplified polymorphic DNA (RAPD) analysis greatly facilitate the epidemiological investigations by means of determining the genetic relationship between strains isolated from a particular outbreak. Examples are given on Pasteurella multocida strains, one of the major pathogens of veterinary importance. Based on the literature and on their own experience the authors recommend the introduction of PFGE and rep-PCR as useful tools for the veterinary diagnostic laboratories.
|Number of pages||6|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Mar 1 2004|
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