DNA amplification polymorphisms of mucor piriformis

Tamás Papp, Csaba Vágvölgyi, Zoltán Kerényi, Ágnes Nagy, Themis J. Michailides

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Abstract

Mucor piriformis can cause postharvest decay in various fruits and vegetables stored at low temperatures. Thirty isolates of this fungus, collected from infected fruit, were subjected to random amplified polymorphic DNA (RAPD) analysis. Seven different 10-bp primers were used to determine the type and extent of intraspecific genetic polymorphisms. Nineteen composite amplification types were identified, indicating a higher degree of variability than found in previous isoenzyme studies. Numerical analysis with the UPGMA technique revealed three clusters, which correlated with the mating competency of the isolates or their place of origin. These results demonstrate that RAPD analysis can identify isolates and subspecific populations of M. piriformis.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume72
Issue number3
DOIs
Publication statusPublished - Nov 24 1997

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Keywords

  • Genetic variability
  • Mating
  • Mucor piriformis
  • Postharvest decay
  • RAPD

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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