Division-site selection, cell separation, and formation of anucleate minicells in Schizosaccharomyces pombe mutants resistant to cell-wall lytic enzymes

A. Grallert, I. Miklós, M. Sipiczki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In most eukaryotic organisms that have cell walls, cell separation or cytokinesis is a degradative enzymatic process. In the fission yeast Schizosaccharormyces pombe, it is a post-M-phase event that includes the degradation of part of the cell wall and the primary septum. We describe the isolation of mutants partially defective in cytokinesis by enrichment of clones resistant to cell-wall lyric enzymes. The mutations confer mycelial morphology (chains of non-separated cells) and define four genes, Sep2-SA2 was subjected to detailed genetic and cytological analysis. Its cells frequently form complex septa composed of multiple layers, which appear as twin septa separated by anucleate minicells if the cell length is extended. This suggests that a polar signal-like mechanism may also operate in S. pombe during division-site selection and sep2+ takes part in it. Sep2+ seems to be involved in several cell cycle functions because its mutation can transiently block cell-cycle progression after nuclear division and provoke a transition from haploidy to diploidy in the double mutant sep2-SA2 cex1-SA2. Cex1 -SA2 is another novel mutation which causes cell-length extension.

Original languageEnglish
Pages (from-to)218-229
Number of pages12
JournalProtoplasma
Volume198
Issue number3-4
Publication statusPublished - 1997

Fingerprint

Schizosaccharomyces
Cell Separation
Schizosaccharomyces pombe
Cell Wall
cell walls
mutants
Cytokinesis
Enzymes
enzymes
Mutation
cytokinesis
Cell Cycle
mutation
cells
Cell Nucleus Division
cell cycle
Haploidy
Diploidy
Cell Division
Clone Cells

Keywords

  • Cell cycle
  • Cell wall
  • Cytokinesis
  • Diploidization
  • Fission yeast
  • Septum

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

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abstract = "In most eukaryotic organisms that have cell walls, cell separation or cytokinesis is a degradative enzymatic process. In the fission yeast Schizosaccharormyces pombe, it is a post-M-phase event that includes the degradation of part of the cell wall and the primary septum. We describe the isolation of mutants partially defective in cytokinesis by enrichment of clones resistant to cell-wall lyric enzymes. The mutations confer mycelial morphology (chains of non-separated cells) and define four genes, Sep2-SA2 was subjected to detailed genetic and cytological analysis. Its cells frequently form complex septa composed of multiple layers, which appear as twin septa separated by anucleate minicells if the cell length is extended. This suggests that a polar signal-like mechanism may also operate in S. pombe during division-site selection and sep2+ takes part in it. Sep2+ seems to be involved in several cell cycle functions because its mutation can transiently block cell-cycle progression after nuclear division and provoke a transition from haploidy to diploidy in the double mutant sep2-SA2 cex1-SA2. Cex1 -SA2 is another novel mutation which causes cell-length extension.",
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T1 - Division-site selection, cell separation, and formation of anucleate minicells in Schizosaccharomyces pombe mutants resistant to cell-wall lytic enzymes

AU - Grallert, A.

AU - Miklós, I.

AU - Sipiczki, M.

PY - 1997

Y1 - 1997

N2 - In most eukaryotic organisms that have cell walls, cell separation or cytokinesis is a degradative enzymatic process. In the fission yeast Schizosaccharormyces pombe, it is a post-M-phase event that includes the degradation of part of the cell wall and the primary septum. We describe the isolation of mutants partially defective in cytokinesis by enrichment of clones resistant to cell-wall lyric enzymes. The mutations confer mycelial morphology (chains of non-separated cells) and define four genes, Sep2-SA2 was subjected to detailed genetic and cytological analysis. Its cells frequently form complex septa composed of multiple layers, which appear as twin septa separated by anucleate minicells if the cell length is extended. This suggests that a polar signal-like mechanism may also operate in S. pombe during division-site selection and sep2+ takes part in it. Sep2+ seems to be involved in several cell cycle functions because its mutation can transiently block cell-cycle progression after nuclear division and provoke a transition from haploidy to diploidy in the double mutant sep2-SA2 cex1-SA2. Cex1 -SA2 is another novel mutation which causes cell-length extension.

AB - In most eukaryotic organisms that have cell walls, cell separation or cytokinesis is a degradative enzymatic process. In the fission yeast Schizosaccharormyces pombe, it is a post-M-phase event that includes the degradation of part of the cell wall and the primary septum. We describe the isolation of mutants partially defective in cytokinesis by enrichment of clones resistant to cell-wall lyric enzymes. The mutations confer mycelial morphology (chains of non-separated cells) and define four genes, Sep2-SA2 was subjected to detailed genetic and cytological analysis. Its cells frequently form complex septa composed of multiple layers, which appear as twin septa separated by anucleate minicells if the cell length is extended. This suggests that a polar signal-like mechanism may also operate in S. pombe during division-site selection and sep2+ takes part in it. Sep2+ seems to be involved in several cell cycle functions because its mutation can transiently block cell-cycle progression after nuclear division and provoke a transition from haploidy to diploidy in the double mutant sep2-SA2 cex1-SA2. Cex1 -SA2 is another novel mutation which causes cell-length extension.

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KW - Cell wall

KW - Cytokinesis

KW - Diploidization

KW - Fission yeast

KW - Septum

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