Diversity in Production of Xylan-Degrading Enzymes Among Species Belonging to the Trichoderma Section Longibrachiatum

Karolina Toth, Martine P. Van Gool, Henk A. Schols, Gary J. Samuels, Harry Gruppen, G. Szakács

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Xylan is an important part of plant biomass and represents a renewable raw material for biorefineries. Contrary to cellulose, the structure of hemicellulose is quite complex. Therefore, the biodegradation of xylan needs the cooperation of many enzymes. For industrial production of xylanase multienzyme complexes (cocktails) and selected monocomponent xylanases, different Trichoderma reesei mutants and recombinants are used. T. reesei QM 6a (wild-type parent of best existing mutants) was selected as a starting material in the 1960s when the modern in-depth analytical methods were not yet in use. Therefore, screening of fungi genetically close to T. reesei in biodegradation of xylan may have a scientific value. Fifteen different strains from Trichoderma section Longibrachiatum have been tested for extracellular xylan-degrading enzyme production on three carbon sources (wheat straw, corn fiber, and eucalyptus wood) in shake flask cultivation. The enzyme activities were evaluated by traditional colorimetric enzyme assays and by HPLC and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Degradation of xylan was studied on four different xylan-rich model substrates. T. reesei CPK 155, Trichoderma parareesei TUB F-2535, and Trichoderma gracile TUB F-2543 isolates were equally good or better in degradation of the wheat arabinoxylan (WAX) and corn fiber alcohol insoluble solids as hydolysis substrates than the well-known T. reesei QM 6a and RUT C30 strains. Though Trichoderma saturnisporum ATCC 18903 gave relatively low volumetric enzyme activities by traditional colorimetric assays, it could release quite large amount of hydrolysis products (mono- and oligosaccharides) from WAX. Therefore, these fungi may be potential candidates for further experiments. Enzyme production on wheat straw and corn fiber carbon sources was more effective than on eucalyptus wood.

Original languageEnglish
Pages (from-to)631-643
Number of pages13
JournalBioenergy Research
Volume6
Issue number2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

xylan
Trichoderma
Trichoderma reesei
Enzymes
enzymes
arabinoxylan
Straw
Enzyme activity
xylanases
wheat straw
Biodegradation
Fungi
biodegradation
Trichoderma saturnisporum
Eucalyptus
corn
Assays
liquid state fermentation
Wood
multienzyme complexes

Keywords

  • Biorefineries
  • Hemicellulase
  • Lignocellulosic biomass
  • Trichoderma
  • Xylan

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Energy (miscellaneous)
  • Renewable Energy, Sustainability and the Environment

Cite this

Diversity in Production of Xylan-Degrading Enzymes Among Species Belonging to the Trichoderma Section Longibrachiatum. / Toth, Karolina; Van Gool, Martine P.; Schols, Henk A.; Samuels, Gary J.; Gruppen, Harry; Szakács, G.

In: Bioenergy Research, Vol. 6, No. 2, 06.2013, p. 631-643.

Research output: Contribution to journalArticle

Toth, Karolina ; Van Gool, Martine P. ; Schols, Henk A. ; Samuels, Gary J. ; Gruppen, Harry ; Szakács, G. / Diversity in Production of Xylan-Degrading Enzymes Among Species Belonging to the Trichoderma Section Longibrachiatum. In: Bioenergy Research. 2013 ; Vol. 6, No. 2. pp. 631-643.
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