Diverse origin of P[19] rotaviruses in children with acute diarrhea in Taiwan: Detection of novel lineages of the G3, G5, and G9 VP7 genes

Fang Tzy Wu, Krisztián Bányai, Jason C. Huang, Ho Sheng Wu, Feng Yee Chang, Jyh Yuan Yang, Chao Agnes Hsiung, Yhu Chering Huang, Jen Shiou Lin, Kao Pin Hwang, Baoming Jiang, Jon R. Gentsch

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We previously reported the detection of genotype P[19] rotavirus strains from children hospitalized with acute dehydrating diarrhea during a 5-year surveillance period in Taiwan. The characterization of five P[19] strains (0.4% of all typed), including three G3P[19], a novel G5P[19], and a unique G9P[19] genotype is described in this study. Phylogenetic analysis of the VP4, VP7, VP6, and NSP4 genes was performed, which demonstrated novel lineages for respective genotypes of the VP4 and the VP7 genes. The sequence similarities of the P[19] VP4 gene among Taiwanese human strains was higher (nt, 91.5-96.2%; aa, 93.7-97.6%) than to other P[19] strains (nt, 83.5-86.6%; aa, 89.4-94.1%) from different regions of the world. The VP7 gene of the three G3P[19] Taiwanese strains shared up to 93.4% nt and 97.5% aa identity to each other but had lower similarity to reference strain sequences available in GenBank (nt, <90.1%; aa, <95.6%). Similarly, the VP7 gene of the novel G5P[19] strain was only moderately related to the VP7 gene of reference G5 strains (nt, 82.2-87.3%; aa, 87.0-93.1%), while the VP7 gene of the single G9P[19] strain was genetically distinct from other known human and animal G9 rotavirus strains (nt, ≤92.0%; aa, ≤95.7%). Together, these findings suggest that the Taiwanese P[19] strains originated by independent interspecies transmission events. Synchronized surveillance of human and animal rotaviruses in Taiwan should identify possible hosts of these uncommon human rotavirus strains.

Original languageEnglish
Pages (from-to)1279-1287
Number of pages9
JournalJournal of Medical Virology
Volume83
Issue number7
DOIs
Publication statusPublished - Jul 1 2011

Keywords

  • NSP4
  • Phylogenetic analysis
  • VP4
  • VP6
  • VP7

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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  • Cite this

    Wu, F. T., Bányai, K., Huang, J. C., Wu, H. S., Chang, F. Y., Yang, J. Y., Hsiung, C. A., Huang, Y. C., Lin, J. S., Hwang, K. P., Jiang, B., & Gentsch, J. R. (2011). Diverse origin of P[19] rotaviruses in children with acute diarrhea in Taiwan: Detection of novel lineages of the G3, G5, and G9 VP7 genes. Journal of Medical Virology, 83(7), 1279-1287. https://doi.org/10.1002/jmv.22052