Diverse effects of a potent LH-RH antagonist on the LH and FSH release.

M. Kovács, M. Koppán, I. Mezó, I. Teplán

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A potent LH-RH antagonist (Ac-D-Trp1,2, D-Cpa2, D-Lys6, D-Ala10 LH-RH (Antag) was used to study the differential regulation of FSH and LH secretion by endogenous LH-RH in ovariectomized (OVX) and regularly cycling rats. The endogenous LH-RH was suppressed by single injections of Antag in OVX animals and by long-term treatment with Antag in normal and OVX rats. Serum and pituitary LH and FSH, as well as serum estradiol (E2) and progesterone (P) was determined by RIA during and/or after the treatment. The direct effect of the antagonistic analog on the ovarian P release was tested in vitro using the isolated luteal cell system. Single injections of the Antag in OVX animals caused prompt and marked suppression of the serum LH (-80%), while no decrease of the serum FSH. Long-term treatment with the same analog decreased the serum LH by 50% but did not modify the serum FSH. In normal rats, serum LH dropped to undetectable levels, while serum FSH did not change significantly. Long-term treatment with the antagonist also resulted in divergent alterations in the pituitary gonadotropin concentrations. In OVX animals, the pituitary LH content moderately elevated (+21%), however, the FSH did not change. In normal rats, ovarian cycles were interrupted, and no ovulation appeared during the treatment. The pituitary LH concentration increased by 46%, while the FSH decreased by 43%. Marked depression was found in the serum P (-60%) but no significant change in the serum E2 levels. Incubation of isolated luteal cells with the Antag did not influence the HCG-induced P secretion in vitro, demonstrating that the in vivo inhibitory effect of the antagonistic LH-RH analog on the P secretion asserts not directly on the ovarian LH-RH receptors, but through inhibition of the endogenous LH-RH. Our studies give evidence that the long-term treatment with LH-RH antagonist suppress the LH and P but not the FSH and E2 secretion, and provide new data suggesting the presence of a FSH-releasing factor in the CNS.

Original languageEnglish
Pages (from-to)285-296
Number of pages12
JournalActa Biologica Hungarica
Volume45
Issue number2-4
Publication statusPublished - 1994

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Gonadotropin-Releasing Hormone
serum
antagonists
secretion
luteal cells
rats
Serum
Rats
pituitary gonadotropins
injection
animals
Animals
Luteal Cells
ovulation
estradiol
progesterone
animal
Pituitary Gonadotropins
Therapeutics
receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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Diverse effects of a potent LH-RH antagonist on the LH and FSH release. / Kovács, M.; Koppán, M.; Mezó, I.; Teplán, I.

In: Acta Biologica Hungarica, Vol. 45, No. 2-4, 1994, p. 285-296.

Research output: Contribution to journalArticle

Kovács, M, Koppán, M, Mezó, I & Teplán, I 1994, 'Diverse effects of a potent LH-RH antagonist on the LH and FSH release.', Acta Biologica Hungarica, vol. 45, no. 2-4, pp. 285-296.
Kovács, M. ; Koppán, M. ; Mezó, I. ; Teplán, I. / Diverse effects of a potent LH-RH antagonist on the LH and FSH release. In: Acta Biologica Hungarica. 1994 ; Vol. 45, No. 2-4. pp. 285-296.
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