Diverse chlamydia-like agents associated with epitheliocystis infection in two cyprinid fish species, the common carp (cyprinus carpio L.) and the gibel carp (carassius auratus gibelio L.)

Boglárka Sellyei, K. Molnár, C. Székely

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

During a general annual fish health survey in natural waters and ponds, epitheliocystis infections were recorded in fingerlings of two cyprinid fish species, the cultured common carp and the wild gibel carp. Benign and heavy infections were equally observed without mortality. In addition to the general health inspection of fish, histopathological examinations of infected gills and molecular biological investigations of separated epitheliocysts were performed. Epitheliocysts were formed both in the interlamellar epithelial cells and in the lamella-free multilayered epithelium of the gill filaments. At the early stage of infection darkstaining inclusion bodies densely stuffed with some pathogenic agents were located at the centre of the cell, while in a progressive stage of the process inclusion bodies within the host cells were disseminated in the cytoplasm and stained pale. Molecular studies demonstrated three different agents related to Neochlamydia, Protochlamydia and Piscichlamydia based on sequence analysis of short regions of the 16S rRNA gene. Among them, Piscichlamydia is a primary fish pathogen, while Neochlamydia and Protochlamydia mostly infect free-living amoebae but have adapted thoroughly to fish.

Original languageEnglish
Pages (from-to)29-40
Number of pages12
JournalActa Veterinaria Hungarica
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Carassius gibelio
Neochlamydia
Goldfish
Chlamydia
Carps
Cyprinus carpio
Fishes
inclusion bodies
fish inspection
gills
Infection
fish
infection
Inclusion Bodies
fish health
Amoeba
fingerlings
epithelial cells
epithelium
sequence analysis

Keywords

  • Chlamydia-like
  • Common carp
  • Epitheliocystis
  • Gibel carp

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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abstract = "During a general annual fish health survey in natural waters and ponds, epitheliocystis infections were recorded in fingerlings of two cyprinid fish species, the cultured common carp and the wild gibel carp. Benign and heavy infections were equally observed without mortality. In addition to the general health inspection of fish, histopathological examinations of infected gills and molecular biological investigations of separated epitheliocysts were performed. Epitheliocysts were formed both in the interlamellar epithelial cells and in the lamella-free multilayered epithelium of the gill filaments. At the early stage of infection darkstaining inclusion bodies densely stuffed with some pathogenic agents were located at the centre of the cell, while in a progressive stage of the process inclusion bodies within the host cells were disseminated in the cytoplasm and stained pale. Molecular studies demonstrated three different agents related to Neochlamydia, Protochlamydia and Piscichlamydia based on sequence analysis of short regions of the 16S rRNA gene. Among them, Piscichlamydia is a primary fish pathogen, while Neochlamydia and Protochlamydia mostly infect free-living amoebae but have adapted thoroughly to fish.",
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AU - Székely, C.

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