Divergent effects of resveratrol, a polyphenolic phytostilbene, on free radical levels and type of cell death induced by the histone deacetylase inhibitors butyrate and trichostatin A

Peter Galfi, Judit Jakus, Tamas Molnar, Susan Neogrady, Adam Csordas

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We investigated the effects of the polyphenolic phytostilbene resveratrol on the steady-state free radical (FR) concentration and mode of cell death induced by the histone deacetylase inhibitors butyrate and trichostatin A. (i) There was no correlation between cell death induction by butyrate or trichostatin A (TSA) and FR levels. (ii) Treatment with resveratrol or N-acetyl-l-cystein (NAC) of cells, in which the FR concentration was high, resulted in an almost complete reduction of FR levels. (iii) When, however, the cellular FR concentration was marginal, resveratrol caused a minor, and NAC a marked increase of FRs as well as of the extent of cell death. Thus, resveratrol and NAC acted as antioxidants only when the cellular FR levels were high, and acted as pro-oxidants when facing a low FR concentration. (iv) Since resveratrol and the antioxidant NAC exhibited analogous effects, it is concluded that the observed actions of resveratrol are due to polyphenolic redox reactions and not related to the stilbene moiety of the molecule. (v) The results indicate that the redox status of a given cell type plays an important role in determining whether resveratrol and other antioxidants promote cell death or protect cells from it.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume94
Issue number1-3 SPEC. ISS.
DOIs
Publication statusPublished - Feb 2005

Keywords

  • Antioxidant
  • Apoptosis
  • Butyrate
  • ESR
  • Free radicals
  • Histone deacetylase inhibitor
  • N-acetylcystein
  • Necrosis
  • Polyphenol
  • Pro-oxidant
  • Resveratrol
  • Stilbene
  • Trichostatin A

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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