Diurnal expression of period 2 and urocortin 1 in neurones of the non-preganglionic Edinger-Westphal nucleus in the rat

B. Gaszner, D. C W A Van Wijk, A. Korosi, R. Józsa, E. W. Roubos, T. Kozicz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Period 2 (Per2) is an important clock gene involved in the regulation of the major circadian clock in the mammalian central nervous system, the suprachiasmatic nucleus. In addition, Per2 is expressed in many other stress-sensitive brain structures. We have previously showed that the non-preganglionic Edinger-Westphal nucleus (npEW) is the main site of the corticotropin-releasing factor peptide family member urocortin 1 (Ucn1) and that this peptide undergoes conspicuous expression changes in response to various stressors. Here, we hypothesized that in the rat npEW both Per2 and Ucn1 would be produced in a diurnal, rhythmical fashion. This hypothesis was tested by following this expected rhythm on two days in rats killed at four time points each day (Zeitgeber times 0, 6, 12, and 18). We showed the co-existence of Per2 and Ucn1 in the npEW with double-label immunofluorescence and demonstrated with quantitative RT-PCR and semi-quantitative immunocytochemistry diurnal rhythms in Per2 mRNA expression and Per2 protein content, each on a single different day, with a minimum at lights-off and a maximum at lights-on. We furthermore revealed a diurnal rhythm in the number of Ucn1-immunopositive neurones and in their Ucn1 peptide content, with a minimum at night and at the beginning of the light period and a peak at lights-off, while the Ucn1 mRNA content paralleled the Per2 mRNA rhythm. The rhythms were accompanied by a diurnal rhythm in plasma corticosterone concentration. Our results are in line with the hypothesis that both Per2 and Ucn1 in the rat npEW are produced in a diurnal fashion, a phenomenon that may be relevant for the regulation of the diurnal rhythm in the stress response.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalStress
Volume12
Issue number2
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Urocortins
Neurons
Circadian Rhythm
Light
Messenger RNA
Peptides
Period Circadian Proteins
Circadian Clocks
Suprachiasmatic Nucleus
Corticotropin-Releasing Hormone
Corticosterone
Edinger-Westphal Nucleus
Fluorescent Antibody Technique
Central Nervous System
Immunohistochemistry
Polymerase Chain Reaction
Brain

Keywords

  • Circadian rhythm
  • Clock gene
  • Edinger-Westphal nucleus
  • Stress
  • Suprachiasmatic nucleus
  • Urocortin

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems
  • Physiology
  • Psychiatry and Mental health
  • Neuropsychology and Physiological Psychology

Cite this

Diurnal expression of period 2 and urocortin 1 in neurones of the non-preganglionic Edinger-Westphal nucleus in the rat. / Gaszner, B.; Van Wijk, D. C W A; Korosi, A.; Józsa, R.; Roubos, E. W.; Kozicz, T.

In: Stress, Vol. 12, No. 2, 03.2009, p. 115-124.

Research output: Contribution to journalArticle

Gaszner, B. ; Van Wijk, D. C W A ; Korosi, A. ; Józsa, R. ; Roubos, E. W. ; Kozicz, T. / Diurnal expression of period 2 and urocortin 1 in neurones of the non-preganglionic Edinger-Westphal nucleus in the rat. In: Stress. 2009 ; Vol. 12, No. 2. pp. 115-124.
@article{57bf4f8216a547bdb252f25a119ddf26,
title = "Diurnal expression of period 2 and urocortin 1 in neurones of the non-preganglionic Edinger-Westphal nucleus in the rat",
abstract = "Period 2 (Per2) is an important clock gene involved in the regulation of the major circadian clock in the mammalian central nervous system, the suprachiasmatic nucleus. In addition, Per2 is expressed in many other stress-sensitive brain structures. We have previously showed that the non-preganglionic Edinger-Westphal nucleus (npEW) is the main site of the corticotropin-releasing factor peptide family member urocortin 1 (Ucn1) and that this peptide undergoes conspicuous expression changes in response to various stressors. Here, we hypothesized that in the rat npEW both Per2 and Ucn1 would be produced in a diurnal, rhythmical fashion. This hypothesis was tested by following this expected rhythm on two days in rats killed at four time points each day (Zeitgeber times 0, 6, 12, and 18). We showed the co-existence of Per2 and Ucn1 in the npEW with double-label immunofluorescence and demonstrated with quantitative RT-PCR and semi-quantitative immunocytochemistry diurnal rhythms in Per2 mRNA expression and Per2 protein content, each on a single different day, with a minimum at lights-off and a maximum at lights-on. We furthermore revealed a diurnal rhythm in the number of Ucn1-immunopositive neurones and in their Ucn1 peptide content, with a minimum at night and at the beginning of the light period and a peak at lights-off, while the Ucn1 mRNA content paralleled the Per2 mRNA rhythm. The rhythms were accompanied by a diurnal rhythm in plasma corticosterone concentration. Our results are in line with the hypothesis that both Per2 and Ucn1 in the rat npEW are produced in a diurnal fashion, a phenomenon that may be relevant for the regulation of the diurnal rhythm in the stress response.",
keywords = "Circadian rhythm, Clock gene, Edinger-Westphal nucleus, Stress, Suprachiasmatic nucleus, Urocortin",
author = "B. Gaszner and {Van Wijk}, {D. C W A} and A. Korosi and R. J{\'o}zsa and Roubos, {E. W.} and T. Kozicz",
year = "2009",
month = "3",
doi = "10.1080/10253890802057221",
language = "English",
volume = "12",
pages = "115--124",
journal = "Stress",
issn = "1025-3890",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Diurnal expression of period 2 and urocortin 1 in neurones of the non-preganglionic Edinger-Westphal nucleus in the rat

AU - Gaszner, B.

AU - Van Wijk, D. C W A

AU - Korosi, A.

AU - Józsa, R.

AU - Roubos, E. W.

AU - Kozicz, T.

PY - 2009/3

Y1 - 2009/3

N2 - Period 2 (Per2) is an important clock gene involved in the regulation of the major circadian clock in the mammalian central nervous system, the suprachiasmatic nucleus. In addition, Per2 is expressed in many other stress-sensitive brain structures. We have previously showed that the non-preganglionic Edinger-Westphal nucleus (npEW) is the main site of the corticotropin-releasing factor peptide family member urocortin 1 (Ucn1) and that this peptide undergoes conspicuous expression changes in response to various stressors. Here, we hypothesized that in the rat npEW both Per2 and Ucn1 would be produced in a diurnal, rhythmical fashion. This hypothesis was tested by following this expected rhythm on two days in rats killed at four time points each day (Zeitgeber times 0, 6, 12, and 18). We showed the co-existence of Per2 and Ucn1 in the npEW with double-label immunofluorescence and demonstrated with quantitative RT-PCR and semi-quantitative immunocytochemistry diurnal rhythms in Per2 mRNA expression and Per2 protein content, each on a single different day, with a minimum at lights-off and a maximum at lights-on. We furthermore revealed a diurnal rhythm in the number of Ucn1-immunopositive neurones and in their Ucn1 peptide content, with a minimum at night and at the beginning of the light period and a peak at lights-off, while the Ucn1 mRNA content paralleled the Per2 mRNA rhythm. The rhythms were accompanied by a diurnal rhythm in plasma corticosterone concentration. Our results are in line with the hypothesis that both Per2 and Ucn1 in the rat npEW are produced in a diurnal fashion, a phenomenon that may be relevant for the regulation of the diurnal rhythm in the stress response.

AB - Period 2 (Per2) is an important clock gene involved in the regulation of the major circadian clock in the mammalian central nervous system, the suprachiasmatic nucleus. In addition, Per2 is expressed in many other stress-sensitive brain structures. We have previously showed that the non-preganglionic Edinger-Westphal nucleus (npEW) is the main site of the corticotropin-releasing factor peptide family member urocortin 1 (Ucn1) and that this peptide undergoes conspicuous expression changes in response to various stressors. Here, we hypothesized that in the rat npEW both Per2 and Ucn1 would be produced in a diurnal, rhythmical fashion. This hypothesis was tested by following this expected rhythm on two days in rats killed at four time points each day (Zeitgeber times 0, 6, 12, and 18). We showed the co-existence of Per2 and Ucn1 in the npEW with double-label immunofluorescence and demonstrated with quantitative RT-PCR and semi-quantitative immunocytochemistry diurnal rhythms in Per2 mRNA expression and Per2 protein content, each on a single different day, with a minimum at lights-off and a maximum at lights-on. We furthermore revealed a diurnal rhythm in the number of Ucn1-immunopositive neurones and in their Ucn1 peptide content, with a minimum at night and at the beginning of the light period and a peak at lights-off, while the Ucn1 mRNA content paralleled the Per2 mRNA rhythm. The rhythms were accompanied by a diurnal rhythm in plasma corticosterone concentration. Our results are in line with the hypothesis that both Per2 and Ucn1 in the rat npEW are produced in a diurnal fashion, a phenomenon that may be relevant for the regulation of the diurnal rhythm in the stress response.

KW - Circadian rhythm

KW - Clock gene

KW - Edinger-Westphal nucleus

KW - Stress

KW - Suprachiasmatic nucleus

KW - Urocortin

UR - http://www.scopus.com/inward/record.url?scp=61349091092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61349091092&partnerID=8YFLogxK

U2 - 10.1080/10253890802057221

DO - 10.1080/10253890802057221

M3 - Article

C2 - 18850494

AN - SCOPUS:61349091092

VL - 12

SP - 115

EP - 124

JO - Stress

JF - Stress

SN - 1025-3890

IS - 2

ER -