Diurnal and circadian variation of protein kinase C immunoreactivity in the rat retina

R. Gábriel, Joseph Lesauter, Rae Silver, Antonio Garcia-Espaa, Paul Witkovsky

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We studied the dependence of the expression of protein kinase C immunoreactivity (PKC-IR) in the rat retina on the light:dark (LD) cycle and on circadian rhythmicity in complete darkness (DD). Two anti-PKC alpha antibodies were employed: One, which we call PKCαβ recognized the hinge region; the other, here termed PKCα, recognized the regulatory region of the molecule. Western blots showed that both anti-PKC antibodies stained an identical single band at approximately 80 kD. The retinal neurons showing PKC-IR were rod bipolar cells and a variety of amacrine neurons. After 3 weeks on an LD cycle, PKCαβ-IR in both rod bipolar and certain amacrine cells manifested a clear rhythm with a peak at zeitgeber time (ZT) of 06-10 hours and a minimum at ZT 18. No rhythm in total PKC-IR was observed when using the PKCα antibody, but, at ZT 06-10 hours, rod bipolar axon terminals showed increased immunostaining. After 48 hours in DD, with either antibody, rod bipolar cells showed increased PKC-IR. The PKCα antibody alone revealed that, after 48 hours, AII amacrine neurons, which lacked PKC-IR in an LD cycle, manifested marked PKC-IR, which became stronger after 72 hours. Light administered early in the dark period greatly increased PKCαβ-IR in rod bipolar and some amacrine neurons. Our data indicate that light and darkness exert a strong regulatory influence on PKC synthesis, activation, and transport in retinal neurons.

Original languageEnglish
Pages (from-to)140-150
Number of pages11
JournalJournal of Comparative Neurology
Volume439
Issue number2
DOIs
Publication statusPublished - Oct 15 2001

Fingerprint

Protein Kinase C
Retina
Photoperiod
Retinal Neurons
Antibodies
Darkness
Neurons
Amacrine Cells
Light
Nucleic Acid Regulatory Sequences
Presynaptic Terminals
Periodicity
Anti-Idiotypic Antibodies
Western Blotting

Keywords

  • Amacrine cell
  • Immunocytochemistry
  • Kinase
  • Rod bipolar cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Diurnal and circadian variation of protein kinase C immunoreactivity in the rat retina. / Gábriel, R.; Lesauter, Joseph; Silver, Rae; Garcia-Espaa, Antonio; Witkovsky, Paul.

In: Journal of Comparative Neurology, Vol. 439, No. 2, 15.10.2001, p. 140-150.

Research output: Contribution to journalArticle

Gábriel, R. ; Lesauter, Joseph ; Silver, Rae ; Garcia-Espaa, Antonio ; Witkovsky, Paul. / Diurnal and circadian variation of protein kinase C immunoreactivity in the rat retina. In: Journal of Comparative Neurology. 2001 ; Vol. 439, No. 2. pp. 140-150.
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