Diuretic and natriuretic actions of extract prepared from rat erythrocytes

L. Selmeci, Elek Pósch, Kinga Kálmán, L. Hársing

Research output: Contribution to journalArticle

Abstract

Freeze-dried preparation of rat erythrocytes was extracted into distilled water and after heat treatment the extract was chromatographed on Sephadex G-25 column. The effluent fractions were pooled, freeze-dried, dissolved in Ringer's solution and bioassayed for diuretic and natriuretic responses in anaesthetized non-diuretic rats. The active fractions potently enhanced urine output (about 9-fold control) and urinary sodium excretion (about 7-fold control). The responses started to develop after a lag period of approximately 10-20 min, peaked between 60-80 min post-injection and then diminished. The augmentation of sodium excretion persisted longer than the increased diuresis. The biological activity of the extract was retained after dialysis in a tube with nominal molecular weight cut-off below 1,000.

Original languageEnglish
Pages (from-to)1025-1031
Number of pages7
JournalLife Sciences
Volume36
Issue number11
DOIs
Publication statusPublished - Mar 18 1985

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Diuretics
Rats
Erythrocytes
Sodium
Dialysis
Diuresis
Bioactivity
Effluents
Hot Temperature
Molecular Weight
Molecular weight
Heat treatment
Urine
Injections
Water
Therapeutics
Ringer's solution
sephadex

ASJC Scopus subject areas

  • Pharmacology

Cite this

Diuretic and natriuretic actions of extract prepared from rat erythrocytes. / Selmeci, L.; Pósch, Elek; Kálmán, Kinga; Hársing, L.

In: Life Sciences, Vol. 36, No. 11, 18.03.1985, p. 1025-1031.

Research output: Contribution to journalArticle

Selmeci, L. ; Pósch, Elek ; Kálmán, Kinga ; Hársing, L. / Diuretic and natriuretic actions of extract prepared from rat erythrocytes. In: Life Sciences. 1985 ; Vol. 36, No. 11. pp. 1025-1031.
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