Diterpenoids and flavonoids from the fruits of Vitex agnus-castus and antioxidant activity of the fruit extracts and their constituents

Zsuzsanna Hajdú, J. Hohmann, P. Forgó, T. Martinek, Máté Dervarics, I. Zupkó, G. Falkay, Daniel Cossuta, I. Máthé

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Abstract

From the n-hexane fraction of the fruits of Vitex agnus-castus, two labdane-type diterpenes, vitetrifolin B and C, were isolated by means of multiple chromatographic separations, together with the previously identified rotundifuran, vitexilactone and the sesquiterpene spathulenol. From the EtOAc fraction, eupatorin was identified for the first time, besides the known casticin, penduletin, vitexin and orientin. The n-hexane, EtOAc and MeOH-H 2O fractions of the MeOH extract of Agni-casti fructus were subjected to in vitro antioxidant assays. The EtOAc extract displayed a significant concentration-dependent effect when tested by 1,1-diphenyl-2-picrylhydrasyl (DPPH) free radical assay (IC50 = 68 μg/mL) and against the autooxidation of a standard rat brain homogenate (IC50 = 14 μg/mL). The MeOH-H2O fraction was less active with 3643 μg/mL (DPPH test) and IC50 = 125 μg/mL (rat brain homogenate), while the n-hexane phase proved to be inactive. The main flavonoid constituents of the EtOAc extract, casticin, vitexin and orientin were assayed for antioxidant activity and found that only casticin possesses a marked lipid peroxidation inhibitory effect (IC50 = 0.049 mM) compared with that of the positive control ascorbic acid (IC50 = 0.703 mM).

Original languageEnglish
Pages (from-to)391-394
Number of pages4
JournalPhytotherapy Research
Volume21
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Vitex
Diterpenes
Fruits
Flavonoids
Inhibitory Concentration 50
Fruit
Antioxidants
Rats
Assays
Brain
Sesquiterpenes
Ascorbic Acid
Free Radicals
Lipids
Lipid Peroxidation
casticin
n-hexane
diphenyl
vitexin
orientin

Keywords

  • Antioxidant activity
  • Flavonoids
  • Labdane diterpenoids
  • Verbenaceae
  • Vitex agnus-castus

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

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title = "Diterpenoids and flavonoids from the fruits of Vitex agnus-castus and antioxidant activity of the fruit extracts and their constituents",
abstract = "From the n-hexane fraction of the fruits of Vitex agnus-castus, two labdane-type diterpenes, vitetrifolin B and C, were isolated by means of multiple chromatographic separations, together with the previously identified rotundifuran, vitexilactone and the sesquiterpene spathulenol. From the EtOAc fraction, eupatorin was identified for the first time, besides the known casticin, penduletin, vitexin and orientin. The n-hexane, EtOAc and MeOH-H 2O fractions of the MeOH extract of Agni-casti fructus were subjected to in vitro antioxidant assays. The EtOAc extract displayed a significant concentration-dependent effect when tested by 1,1-diphenyl-2-picrylhydrasyl (DPPH) free radical assay (IC50 = 68 μg/mL) and against the autooxidation of a standard rat brain homogenate (IC50 = 14 μg/mL). The MeOH-H2O fraction was less active with 3643 μg/mL (DPPH test) and IC50 = 125 μg/mL (rat brain homogenate), while the n-hexane phase proved to be inactive. The main flavonoid constituents of the EtOAc extract, casticin, vitexin and orientin were assayed for antioxidant activity and found that only casticin possesses a marked lipid peroxidation inhibitory effect (IC50 = 0.049 mM) compared with that of the positive control ascorbic acid (IC50 = 0.703 mM).",
keywords = "Antioxidant activity, Flavonoids, Labdane diterpenoids, Verbenaceae, Vitex agnus-castus",
author = "Zsuzsanna Hajd{\'u} and J. Hohmann and P. Forg{\'o} and T. Martinek and M{\'a}t{\'e} Dervarics and I. Zupk{\'o} and G. Falkay and Daniel Cossuta and I. M{\'a}th{\'e}",
year = "2007",
month = "4",
doi = "10.1002/ptr.2021",
language = "English",
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TY - JOUR

T1 - Diterpenoids and flavonoids from the fruits of Vitex agnus-castus and antioxidant activity of the fruit extracts and their constituents

AU - Hajdú, Zsuzsanna

AU - Hohmann, J.

AU - Forgó, P.

AU - Martinek, T.

AU - Dervarics, Máté

AU - Zupkó, I.

AU - Falkay, G.

AU - Cossuta, Daniel

AU - Máthé, I.

PY - 2007/4

Y1 - 2007/4

N2 - From the n-hexane fraction of the fruits of Vitex agnus-castus, two labdane-type diterpenes, vitetrifolin B and C, were isolated by means of multiple chromatographic separations, together with the previously identified rotundifuran, vitexilactone and the sesquiterpene spathulenol. From the EtOAc fraction, eupatorin was identified for the first time, besides the known casticin, penduletin, vitexin and orientin. The n-hexane, EtOAc and MeOH-H 2O fractions of the MeOH extract of Agni-casti fructus were subjected to in vitro antioxidant assays. The EtOAc extract displayed a significant concentration-dependent effect when tested by 1,1-diphenyl-2-picrylhydrasyl (DPPH) free radical assay (IC50 = 68 μg/mL) and against the autooxidation of a standard rat brain homogenate (IC50 = 14 μg/mL). The MeOH-H2O fraction was less active with 3643 μg/mL (DPPH test) and IC50 = 125 μg/mL (rat brain homogenate), while the n-hexane phase proved to be inactive. The main flavonoid constituents of the EtOAc extract, casticin, vitexin and orientin were assayed for antioxidant activity and found that only casticin possesses a marked lipid peroxidation inhibitory effect (IC50 = 0.049 mM) compared with that of the positive control ascorbic acid (IC50 = 0.703 mM).

AB - From the n-hexane fraction of the fruits of Vitex agnus-castus, two labdane-type diterpenes, vitetrifolin B and C, were isolated by means of multiple chromatographic separations, together with the previously identified rotundifuran, vitexilactone and the sesquiterpene spathulenol. From the EtOAc fraction, eupatorin was identified for the first time, besides the known casticin, penduletin, vitexin and orientin. The n-hexane, EtOAc and MeOH-H 2O fractions of the MeOH extract of Agni-casti fructus were subjected to in vitro antioxidant assays. The EtOAc extract displayed a significant concentration-dependent effect when tested by 1,1-diphenyl-2-picrylhydrasyl (DPPH) free radical assay (IC50 = 68 μg/mL) and against the autooxidation of a standard rat brain homogenate (IC50 = 14 μg/mL). The MeOH-H2O fraction was less active with 3643 μg/mL (DPPH test) and IC50 = 125 μg/mL (rat brain homogenate), while the n-hexane phase proved to be inactive. The main flavonoid constituents of the EtOAc extract, casticin, vitexin and orientin were assayed for antioxidant activity and found that only casticin possesses a marked lipid peroxidation inhibitory effect (IC50 = 0.049 mM) compared with that of the positive control ascorbic acid (IC50 = 0.703 mM).

KW - Antioxidant activity

KW - Flavonoids

KW - Labdane diterpenoids

KW - Verbenaceae

KW - Vitex agnus-castus

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U2 - 10.1002/ptr.2021

DO - 10.1002/ptr.2021

M3 - Article

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AN - SCOPUS:34247151652

VL - 21

SP - 391

EP - 394

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 4

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