Disubstituted dialkoxysilane precursors in binary and ternary sol-gel systems for lipase immobilization

Diána Weiser, Zoltán Boros, Gábor Hornyánszky, Alexandra Tóth, L. Poppe

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The disubstituted dimethyldiethoxysilane (DMDEOS), methyl(phenyl) diethoxysilane (MPDEOS) and diphenyldiethoxysilane (DPDEOS) were used in binary silane precursor systems in combination with tetraethoxysilane (TEOS) for the immobilization of lipase from Pseudomonas fluorescens (Lipase AK). In addition, ternary silane precursor systems with TEOS and octyltriethoxysilane (OTEOS) or phenyltriethoxysilane (PTEOS) were also studied for encapsulation. The best performing ternary sol-gel preparations (418-736% activity yields of the immobilized enzyme with 1-phenylethanol rac-1a as compared to the native form) were tested as biocatalysts for kinetic resolutions of rac-1a, 1-phenylpropan-2-ol rac-1b and 4-phenylbutan-2-ol rac-1c. Because the catalytic properties and the operational stability of the DMDEOS-containing preparations proved to be superior to all the tested free and sol-gel entrapped Lipase AK biocatalysts in batch mode, the kinetic resolutions of rac-1a and rac-1b were performed with the TEOS/PTEOS/DMDEOS 4:1:1 Lipase AK in a continuous-flow reactor as well.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalProcess Biochemistry
Volume47
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Lipases
Polymethyl Methacrylate
Lipase
Immobilization
Sol-gels
Gels
Silanes
Biocatalysts
Polymers
Enzyme kinetics
Pseudomonas fluorescens
Immobilized Enzymes
Kinetics
Enzymes
Encapsulation
tetraethoxysilane
phenyltriethoxysilane

Keywords

  • Continuous-flow reactor
  • Disubstituted dialkoxysilane
  • Immobilization
  • Kinetic resolution
  • Lipase
  • Sol-gel

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Disubstituted dialkoxysilane precursors in binary and ternary sol-gel systems for lipase immobilization. / Weiser, Diána; Boros, Zoltán; Hornyánszky, Gábor; Tóth, Alexandra; Poppe, L.

In: Process Biochemistry, Vol. 47, No. 3, 03.2012, p. 428-434.

Research output: Contribution to journalArticle

Weiser, Diána ; Boros, Zoltán ; Hornyánszky, Gábor ; Tóth, Alexandra ; Poppe, L. / Disubstituted dialkoxysilane precursors in binary and ternary sol-gel systems for lipase immobilization. In: Process Biochemistry. 2012 ; Vol. 47, No. 3. pp. 428-434.
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