Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease

György Baffy, Z. Varga, Gabriella Fóris, András Leövey

Research output: Contribution to journalArticle

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Abstract

Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration ([Ca2+]1) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]1 rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]1 levels, and the diminished [Ca2+]1 elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2+-ATPase.

Original languageEnglish
Pages (from-to)327-334
Number of pages8
JournalClinical Biochemistry
Volume23
Issue number4
Publication statusPublished - 1990

Fingerprint

Alcoholic Liver Diseases
Liver
Hepatocytes
Neutrophils
Calcium
N-Formylmethionine Leucyl-Phenylalanine
Trifluoperazine
Calcium-Transporting ATPases
Calmodulin
Cell membranes
LDL Lipoproteins
Extrusion
Cell Membrane

Keywords

  • Alcoholic liver disease
  • ATPase
  • Calcium efflux
  • Intracellular free calcium
  • Isolated human hepatocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease. / Baffy, György; Varga, Z.; Fóris, Gabriella; Leövey, András.

In: Clinical Biochemistry, Vol. 23, No. 4, 1990, p. 327-334.

Research output: Contribution to journalArticle

Baffy, György ; Varga, Z. ; Fóris, Gabriella ; Leövey, András. / Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease. In: Clinical Biochemistry. 1990 ; Vol. 23, No. 4. pp. 327-334.
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