Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease

György Baffy, Z. Varga, Gabriella Fóris, András Leövey

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration ([Ca2+]l) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]l rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]l levels, and the diminished [Ca2+]l elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2+-ATPase.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalClinical Biochemistry
Volume23
Issue number3
DOIs
Publication statusPublished - 1990

Fingerprint

Alcoholic Liver Diseases
Liver
Hepatocytes
Neutrophils
Calcium
N-Formylmethionine Leucyl-Phenylalanine
Trifluoperazine
Calcium-Transporting ATPases
Calmodulin
Cell membranes
LDL Lipoproteins
Extrusion
Cell Membrane

Keywords

  • alcoholic liver disease
  • ATPase
  • calcium efflux
  • intracellular free calcium
  • isolated human hepatocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease. / Baffy, György; Varga, Z.; Fóris, Gabriella; Leövey, András.

In: Clinical Biochemistry, Vol. 23, No. 3, 1990, p. 241-245.

Research output: Contribution to journalArticle

Baffy, György ; Varga, Z. ; Fóris, Gabriella ; Leövey, András. / Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease. In: Clinical Biochemistry. 1990 ; Vol. 23, No. 3. pp. 241-245.
@article{0a369aeafc79493a80a264adeba3aebc,
title = "Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease",
abstract = "Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration ([Ca2+]l) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]l rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]l levels, and the diminished [Ca2+]l elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2+-ATPase.",
keywords = "alcoholic liver disease, ATPase, calcium efflux, intracellular free calcium, isolated human hepatocytes",
author = "Gy{\"o}rgy Baffy and Z. Varga and Gabriella F{\'o}ris and Andr{\'a}s Le{\"o}vey",
year = "1990",
doi = "10.1016/0009-9120(90)90692-N",
language = "English",
volume = "23",
pages = "241--245",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease

AU - Baffy, György

AU - Varga, Z.

AU - Fóris, Gabriella

AU - Leövey, András

PY - 1990

Y1 - 1990

N2 - Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration ([Ca2+]l) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]l rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]l levels, and the diminished [Ca2+]l elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2+-ATPase.

AB - Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration ([Ca2+]l) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]l rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]l levels, and the diminished [Ca2+]l elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2+-ATPase.

KW - alcoholic liver disease

KW - ATPase

KW - calcium efflux

KW - intracellular free calcium

KW - isolated human hepatocytes

UR - http://www.scopus.com/inward/record.url?scp=0025372534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025372534&partnerID=8YFLogxK

U2 - 10.1016/0009-9120(90)90692-N

DO - 10.1016/0009-9120(90)90692-N

M3 - Article

VL - 23

SP - 241

EP - 245

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

IS - 3

ER -