Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease

György Baffy, Zsuzsa Varga, Gabriella Fóris, András Leövey

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Abstract

Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration ([Ca2+]l) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]l rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]l levels, and the diminished [Ca2+]l elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2+-ATPase.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalClinical Biochemistry
Volume23
Issue number3
DOIs
Publication statusPublished - Jun 1990

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Keywords

  • ATPase
  • alcoholic liver disease
  • calcium efflux
  • intracellular free calcium
  • isolated human hepatocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

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