Disturbed brain purine metabolism results in a gross opening of the blood-brain barrier in newborn piglets following experimental pneumothorax

Péter Temesvári, C. Ábrahám, Ferenc Joó, József Kovács, Zsuzsa Baranyai, Katalin Rácz

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Abstract

Changes in the permeability of pial-arachnoideal microvessels [30-210 μm), of the blood-brain barrier (BBB), were intravitally studied by fluorescent microscopy and compared to the hypoxanthine (HX) level of cerebrospinal fluid (CSF) in newborn piglets (n = 24) using the open cranial window technique. Eight animals served as controls (Group 1), the others were studied in the course of bilateral experimental pneumothorax (BEP) using low (Na+-fluorescein, MW 376, Group 2) and large molecular weight (FITC dextran, MW 40 000, Group 3) fluorescent tracer molecules. Cisternal CSF was sampled from the animals: 8 piglets from Group 1, and 4-4 piglets from Groups 2 and 3 at different stages of pathological condition: (i) at the critical (C) stage (severe acidosis, bradycardia, arterial hypotension and hypoxaemia) and also (ii) at the recovery (R) stage (mild metabolic acidosis, tachycardia, arterial hypotension) and the HX concentration was determined with high-pressure liquid chromatography. In Group 1 neither low (n = 4) nor large (n = 4) molecular weight tracers penetrated BBB. In Group 2, however, the fluorescein dye passed BBB as a spotty leakage in animals at C stage (n = 8). Diffuse fluorescein penetration was seen at R stage, too (n = 4). In Group 3 no change in permeability was found at C stages (n = 8), but at R stage (n = 4), 2h after the primary hypoxic insult, when the animals had recovered from cardiovascular and metabolic shock, the tracer passed locally the microvascular wall and appeared as leaky spots (number of leaky sites = 2.3 ± 0.4 0.10 cm2, X ̄ ± SE. The HX content in the CSF was significantly elevated (P <0.05) at stage C (26.39 ± 3.2 μM/l, X ̄, ± SE, compared to data measured in Group 1 (8.4 ± 1.7 μM/l, X ̄ ± SE. At stage R, it proved to be significantly lower (P <0.05) than in stage C, but still remained elevated (17.7 ± 2.1 μM/l, X ̄ ± SE; P <0.05 vs. Group 1). Our results draw the attention of the size-dependent gross opening of the BBB in different stages of BEP and to the possible importance in the pathogenesis of mediators deriving from the disturbed brain purine metabolism.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalNeuroscience Letters
Volume113
Issue number2
DOIs
Publication statusPublished - May 31 1990

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Pneumothorax
Blood-Brain Barrier
Hypoxanthine
Fluorescein
Cerebrospinal Fluid
Brain
Acidosis
Hypotension
Permeability
Molecular Weight
Bradycardia
Microvessels
Dextrans
Tachycardia
Microscopy
Shock
Coloring Agents
High Pressure Liquid Chromatography
Control Groups
purine

Keywords

  • Brain microvessel
  • CSF hypoxanthine
  • Intravital fluorescence microscopy
  • Piglet
  • Pneumothorax

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Disturbed brain purine metabolism results in a gross opening of the blood-brain barrier in newborn piglets following experimental pneumothorax. / Temesvári, Péter; Ábrahám, C.; Joó, Ferenc; Kovács, József; Baranyai, Zsuzsa; Rácz, Katalin.

In: Neuroscience Letters, Vol. 113, No. 2, 31.05.1990, p. 163-168.

Research output: Contribution to journalArticle

Temesvári, Péter ; Ábrahám, C. ; Joó, Ferenc ; Kovács, József ; Baranyai, Zsuzsa ; Rácz, Katalin. / Disturbed brain purine metabolism results in a gross opening of the blood-brain barrier in newborn piglets following experimental pneumothorax. In: Neuroscience Letters. 1990 ; Vol. 113, No. 2. pp. 163-168.
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AU - Joó, Ferenc

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