Distribution of tuberoinfundibular peptide of 39 residues and its receptor, parathyroid hormone 2 receptor, in the mouse brain

Catherine A. Faber, A. Dobolyi, Mark Sleeman, Ted B. Usdin

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Tuberoinfundibular peptide of 39 residues (TIP39) was identified as a potent parathyroid hormone 2 receptor (PTH2R) agonist. Existing anatomical data also support the suggestion that TIP39 is the PTH2R's endogenous ligand, but a comprehensive comparison of TIP39 and PTH2R distributions has not been performed. In the present study, we compared the distributions of TIP39 and PTH2R on adjacent mouse brain sections. In addition, we determined the locations of PTH2R-expressing cell bodies by in situ hybridization histochemistry and by labeling β-galactosidase driven by the PTH2R promoter in knockin mice. An excellent correlation was found between the distributions of TIP39-containing fibers and PTH2R-containing cell bodies and fibers throughout the brain. TIP39 and the PTH2R are abundant in medial prefrontal, insular, and ectorhinal cortices, the lateral septal nucleus, the bed nucleus of the stria terminalis, the fundus striati, the amygdala, the ventral subiculum, the hypothalamus, midline and intralaminar thalamic nuclei, the medial geniculate body, the periaqueductal gray, the ventral tegmental area, the superior and inferior colliculi, the parabrachial nuclei, the locus coeruleus, subcoeruleus and periolivary areas, and the nucleus of the solitary tract. Furthermore, even the subregional distribution of TIP39- and PTH2R-immunoreactive fibers in these regions showed remarkable similarities, providing anatomical evidence that TIP39 may act on the PTH2R. Based on these observations and on previous pharmacological data, we propose that TIP39 is an endogenous ligand of the PTH2R and that they form a neuromodulator system, which is optimally positioned to regulate limbic, endocrine, and auditory brain functions.

Original languageEnglish
Pages (from-to)563-583
Number of pages21
JournalJournal of Comparative Neurology
Volume502
Issue number4
DOIs
Publication statusPublished - Jun 1 2007

Fingerprint

Receptor, Parathyroid Hormone, Type 2
Brain
Septal Nuclei
Midline Thalamic Nuclei
Parathyroid Hormone Receptor Type 1
Galactosidases
Intralaminar Thalamic Nuclei
Geniculate Bodies
Ligands
tuberoinfundibular peptide 39
Inferior Colliculi
Periaqueductal Gray
Ventral Tegmental Area
Solitary Nucleus
Locus Coeruleus
Superior Colliculi
Amygdala
Cerebral Cortex
Hypothalamus
In Situ Hybridization

Keywords

  • Neuroanatomical distribution
  • Neuromodulator
  • Neuropeptide
  • Parathyroid hormone 2 receptor
  • Promoter driven expression
  • TIP39 and PTH2 receptor in situ hybridization and immunocytochemistry
  • Transgenic mouse
  • Tuberoinfundibular peptide of 39 residues

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of tuberoinfundibular peptide of 39 residues and its receptor, parathyroid hormone 2 receptor, in the mouse brain. / Faber, Catherine A.; Dobolyi, A.; Sleeman, Mark; Usdin, Ted B.

In: Journal of Comparative Neurology, Vol. 502, No. 4, 01.06.2007, p. 563-583.

Research output: Contribution to journalArticle

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