Distribution of secretin receptors in the rat central nervous system

An in situ hybridization study

Z. Tóth, Andrea Heinzlmann, Hitoshi Hashimoto, K. Köves

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Secretin shows a wide distribution in the brain. Functional significance of central secretin is stressed since it has been associated with autism and schizophrenia. The presence of the secretin receptor was previously demonstrated in the brain by different methods. Neurons in the cerebellum, hypothalamic paraventricular and supraoptic nuclei, and in the vascular organ of lamina terminalis were shown to express secretin receptor mRNA by using in situ hybridization with digoxigenin-labeled probe. In this work, we used a very sensitive radioactive in situ hybridization technique and systematically mapped the expression of secretin receptor mRNA in the brain. The densest labeling was observed in the nucleus of solitary tract and in the laterodorsal thalamic nucleus, where decreasing number of receptors was seen in the vascular organ of lamina terminalis, and the lateral habenular complex, and then in the supraoptic nucleus. Only a few scattered labeled cells were observed in the median frontal gyrus, entorhinal cortex, hypothalamic paraventricular nucleus, perifornical region, lateral hypothalamic area, head of the caudate nucleus, spinal trigeminal nucleus, and cerebellum. Secretin receptor mRNA showed a far wider distribution than was known before, suggesting a more significant functional relevance than thought earlier.

Original languageEnglish
Pages (from-to)172-178
Number of pages7
JournalJournal of Molecular Neuroscience
Volume50
Issue number1
DOIs
Publication statusPublished - May 2013

Fingerprint

In Situ Hybridization
Central Nervous System
Supraoptic Nucleus
Secretin
Paraventricular Hypothalamic Nucleus
Cerebellum
Messenger RNA
Hypothalamus
Blood Vessels
Brain
Spinal Trigeminal Nucleus
Lateral Hypothalamic Area
Lateral Thalamic Nuclei
Digoxigenin
Entorhinal Cortex
Solitary Nucleus
Caudate Nucleus
Autistic Disorder
Schizophrenia
Neurons

Keywords

  • Brain stem
  • Forebrain
  • In vitro transcription
  • Mapping
  • Radioactive labeling

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Distribution of secretin receptors in the rat central nervous system : An in situ hybridization study. / Tóth, Z.; Heinzlmann, Andrea; Hashimoto, Hitoshi; Köves, K.

In: Journal of Molecular Neuroscience, Vol. 50, No. 1, 05.2013, p. 172-178.

Research output: Contribution to journalArticle

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