Distribution of peripheral blood cells in mice after splenectomy or autotransplantation

Sandor Sipka, E. Bráth, Ferenc F. Toth, Akos Fabian, Csilla Krizsan, S. Baráth, Sandor Sipka, N. Németh, Anita Balint, I. Furka, I. Mikó

Research output: Contribution to journalArticle

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Abstract

Our aim was to compare the distribution changes of peripheral leukocytes and erythrocytes in splenectomized and spleen-autotransplanted BALB/c female mice (n = 96), 2 and 8 months after surgery. In total, there were eight groups of animals: splenectomy, autotransplantation, sham, and untreated controls at both time points. We used the spleen-apron method of Furka et al. (Khirurgiia (Mosk) 1989;9:125-127), inserting five spleen chips into the greater omentum, for autotransplantation. Quantitative and qualitative blood cell counts and the phagocytic activity of cells (stimulated with zymosan) were determined. In splenectomized animals, the number of neutrophils significantly increased 8 months after surgery. The greatest phagocytic activity of neutrophils, however, was observed in autotransplanted animals of the same age. In splenectomized animals, erythrocyte volumes were significantly higher in the second postoperative month, but normalized by the eighth month. In conclusion, spleen autotransplantation has some beneficial effects, including clearing erythrocytes and preserving the phagocytic activity of neutrophils in peripheral blood.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalMicrosurgery
Volume26
Issue number1
DOIs
Publication statusPublished - 2006

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Autologous Transplantation
Splenectomy
Blood Cells
Spleen
Neutrophils
Erythrocytes
Erythrocyte Volume
Omentum
Zymosan
Blood Cell Count
Phagocytes
Leukocytes

ASJC Scopus subject areas

  • Surgery

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Distribution of peripheral blood cells in mice after splenectomy or autotransplantation. / Sipka, Sandor; Bráth, E.; Toth, Ferenc F.; Fabian, Akos; Krizsan, Csilla; Baráth, S.; Sipka, Sandor; Németh, N.; Balint, Anita; Furka, I.; Mikó, I.

In: Microsurgery, Vol. 26, No. 1, 2006, p. 43-49.

Research output: Contribution to journalArticle

Sipka, Sandor ; Bráth, E. ; Toth, Ferenc F. ; Fabian, Akos ; Krizsan, Csilla ; Baráth, S. ; Sipka, Sandor ; Németh, N. ; Balint, Anita ; Furka, I. ; Mikó, I. / Distribution of peripheral blood cells in mice after splenectomy or autotransplantation. In: Microsurgery. 2006 ; Vol. 26, No. 1. pp. 43-49.
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