Distribution of parathyroid hormone-2 receptor-like immunoreactivity and messenger RNA in the rat nervous system

T. Wang, M. Palkóvits, M. Rusnak, É Mezey, T. B. Usdin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The parathyroid hormone-2 receptor is a member of the secretin family of guanine nucleotide-binding protein-coupled receptors. The human parathyroid hormone-2 receptor is activated by parathyroid hormone and a recently purified hypothalamic polypeptide, tubero-infundibular peptide of 39 residues, while the rat parathyroid hormone-2 receptor is poorly activated by parathyroid hormone and is potently activated by tubero-infundibular peptide of 39 residues. In order to provide a foundation for studies on the physiological role of the parathyroid hormone-2 receptor and tubero-infundibular peptide of 39 residues, we investigated the cellular distribution of the parathyroid hormone-2 receptor in the rat CNS using both immunohistochemistry and in situ hybridization histochemistry. The receptor is found in discrete groups of neurons in many regions. It is present in scattered small cells throughout the cerebral cortex, in small and medium-sized cells in the striatum, and is quite abundant in the septum and the midline thalamic nuclei. Its expression is high in the hypothalamus, particularly in the periventricular and arcuate nuclei. Fibers and terminals in the external zone of the median eminence, and in the superficial layers of the caudal spinal trigeminal tract and the spinal cord dorsal horn, are strongly and dramatically labeled by a parathyroid hormone-2 receptor-selective antibody.The localization of parathyroid hormone-2 receptor suggests a role in the regulation of pituitary hormone secretion, sensory information processing and homeostatic regulation. Copyright (C) 2000.

Original languageEnglish
Pages (from-to)629-649
Number of pages21
JournalNeuroscience
Volume100
Issue number3
DOIs
Publication statusPublished - Sep 27 2000

Fingerprint

Receptor, Parathyroid Hormone, Type 2
Nervous System
Messenger RNA
Peptides
Parathyroid Hormone
Midline Thalamic Nuclei
Median Eminence
Arcuate Nucleus of Hypothalamus
Secretin
Guanine Nucleotides
Pituitary Hormones
Automatic Data Processing
Cerebral Cortex
Hypothalamus
In Situ Hybridization
Carrier Proteins
Immunohistochemistry
Neurons

Keywords

  • Anatomical localization
  • Immunohistochemistry
  • In situ hybridization
  • Neuropeptide receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of parathyroid hormone-2 receptor-like immunoreactivity and messenger RNA in the rat nervous system. / Wang, T.; Palkóvits, M.; Rusnak, M.; Mezey, É; Usdin, T. B.

In: Neuroscience, Vol. 100, No. 3, 27.09.2000, p. 629-649.

Research output: Contribution to journalArticle

Wang, T. ; Palkóvits, M. ; Rusnak, M. ; Mezey, É ; Usdin, T. B. / Distribution of parathyroid hormone-2 receptor-like immunoreactivity and messenger RNA in the rat nervous system. In: Neuroscience. 2000 ; Vol. 100, No. 3. pp. 629-649.
@article{ef867a30551e4511a1901acbda684e0a,
title = "Distribution of parathyroid hormone-2 receptor-like immunoreactivity and messenger RNA in the rat nervous system",
abstract = "The parathyroid hormone-2 receptor is a member of the secretin family of guanine nucleotide-binding protein-coupled receptors. The human parathyroid hormone-2 receptor is activated by parathyroid hormone and a recently purified hypothalamic polypeptide, tubero-infundibular peptide of 39 residues, while the rat parathyroid hormone-2 receptor is poorly activated by parathyroid hormone and is potently activated by tubero-infundibular peptide of 39 residues. In order to provide a foundation for studies on the physiological role of the parathyroid hormone-2 receptor and tubero-infundibular peptide of 39 residues, we investigated the cellular distribution of the parathyroid hormone-2 receptor in the rat CNS using both immunohistochemistry and in situ hybridization histochemistry. The receptor is found in discrete groups of neurons in many regions. It is present in scattered small cells throughout the cerebral cortex, in small and medium-sized cells in the striatum, and is quite abundant in the septum and the midline thalamic nuclei. Its expression is high in the hypothalamus, particularly in the periventricular and arcuate nuclei. Fibers and terminals in the external zone of the median eminence, and in the superficial layers of the caudal spinal trigeminal tract and the spinal cord dorsal horn, are strongly and dramatically labeled by a parathyroid hormone-2 receptor-selective antibody.The localization of parathyroid hormone-2 receptor suggests a role in the regulation of pituitary hormone secretion, sensory information processing and homeostatic regulation. Copyright (C) 2000.",
keywords = "Anatomical localization, Immunohistochemistry, In situ hybridization, Neuropeptide receptor",
author = "T. Wang and M. Palk{\'o}vits and M. Rusnak and {\'E} Mezey and Usdin, {T. B.}",
year = "2000",
month = "9",
day = "27",
doi = "10.1016/S0306-4522(00)00282-7",
language = "English",
volume = "100",
pages = "629--649",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Distribution of parathyroid hormone-2 receptor-like immunoreactivity and messenger RNA in the rat nervous system

AU - Wang, T.

AU - Palkóvits, M.

AU - Rusnak, M.

AU - Mezey, É

AU - Usdin, T. B.

PY - 2000/9/27

Y1 - 2000/9/27

N2 - The parathyroid hormone-2 receptor is a member of the secretin family of guanine nucleotide-binding protein-coupled receptors. The human parathyroid hormone-2 receptor is activated by parathyroid hormone and a recently purified hypothalamic polypeptide, tubero-infundibular peptide of 39 residues, while the rat parathyroid hormone-2 receptor is poorly activated by parathyroid hormone and is potently activated by tubero-infundibular peptide of 39 residues. In order to provide a foundation for studies on the physiological role of the parathyroid hormone-2 receptor and tubero-infundibular peptide of 39 residues, we investigated the cellular distribution of the parathyroid hormone-2 receptor in the rat CNS using both immunohistochemistry and in situ hybridization histochemistry. The receptor is found in discrete groups of neurons in many regions. It is present in scattered small cells throughout the cerebral cortex, in small and medium-sized cells in the striatum, and is quite abundant in the septum and the midline thalamic nuclei. Its expression is high in the hypothalamus, particularly in the periventricular and arcuate nuclei. Fibers and terminals in the external zone of the median eminence, and in the superficial layers of the caudal spinal trigeminal tract and the spinal cord dorsal horn, are strongly and dramatically labeled by a parathyroid hormone-2 receptor-selective antibody.The localization of parathyroid hormone-2 receptor suggests a role in the regulation of pituitary hormone secretion, sensory information processing and homeostatic regulation. Copyright (C) 2000.

AB - The parathyroid hormone-2 receptor is a member of the secretin family of guanine nucleotide-binding protein-coupled receptors. The human parathyroid hormone-2 receptor is activated by parathyroid hormone and a recently purified hypothalamic polypeptide, tubero-infundibular peptide of 39 residues, while the rat parathyroid hormone-2 receptor is poorly activated by parathyroid hormone and is potently activated by tubero-infundibular peptide of 39 residues. In order to provide a foundation for studies on the physiological role of the parathyroid hormone-2 receptor and tubero-infundibular peptide of 39 residues, we investigated the cellular distribution of the parathyroid hormone-2 receptor in the rat CNS using both immunohistochemistry and in situ hybridization histochemistry. The receptor is found in discrete groups of neurons in many regions. It is present in scattered small cells throughout the cerebral cortex, in small and medium-sized cells in the striatum, and is quite abundant in the septum and the midline thalamic nuclei. Its expression is high in the hypothalamus, particularly in the periventricular and arcuate nuclei. Fibers and terminals in the external zone of the median eminence, and in the superficial layers of the caudal spinal trigeminal tract and the spinal cord dorsal horn, are strongly and dramatically labeled by a parathyroid hormone-2 receptor-selective antibody.The localization of parathyroid hormone-2 receptor suggests a role in the regulation of pituitary hormone secretion, sensory information processing and homeostatic regulation. Copyright (C) 2000.

KW - Anatomical localization

KW - Immunohistochemistry

KW - In situ hybridization

KW - Neuropeptide receptor

UR - http://www.scopus.com/inward/record.url?scp=0034721504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034721504&partnerID=8YFLogxK

U2 - 10.1016/S0306-4522(00)00282-7

DO - 10.1016/S0306-4522(00)00282-7

M3 - Article

C2 - 11098126

AN - SCOPUS:0034721504

VL - 100

SP - 629

EP - 649

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 3

ER -