Distribution of nociceptin in pancreatic islet cells of normal and diabetic rats

Saeed Tariq, Hameed Rashed, Syed M. Nurulain, Bright Starling Emerald, Surya Koturan, K. Tekes, Ernest Adeghate

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives Nociceptin has been reported to play an important role in the regulation of pancreatic exocrine secretion. Most of the studies performed on nociceptin are mainly physiological rather than morphological in nature. The present study investigated the pattern of distribution of nociceptin in the endocrine pancreas of normal and diabetic rats. Methods Immunohistochemistry, immunofluorescence, Western blot, and double-labeled immunoelectron microscopy were used in this study. Diabetes was induced using streptozotocin (60 mg/kg body weight). Results Nociceptin-immunoreactive cells were observed in the central and peripheral regions of the islets of both normal and diabetic rat pancreas. The number of nociceptin-positive cells was significantly (P <0.05) lower in the islet of diabetic rats compared with the control. Immunofluorescence study showed that nociceptin colocalizes with insulin in pancreatic β-cells. The degree of colocalization of nociceptin with insulin was severely deranged after the onset of diabetes. Moreover, immunogold particles conjugated with either nociceptin or insulin were observed on the granules of pancreatic β-cell. The number of nociceptin-labeled colloidal gold particles was significantly lower after the onset of diabetes. Conclusions Nociceptin is present in pancreatic islets cells and colocalizes with insulin. Nociceptin may have a physiological role in the metabolism of insulin.

Original languageEnglish
Pages (from-to)602-607
Number of pages6
JournalPancreas.
Volume44
Issue number4
DOIs
Publication statusPublished - May 25 2015

Fingerprint

Islets of Langerhans
Insulin
Fluorescent Antibody Technique
nociceptin
Gold Colloid
Immunoelectron Microscopy
Streptozocin
Pancreas
Western Blotting
Immunohistochemistry
Body Weight

Keywords

  • diabetes mellitus
  • islet cells
  • nociceptin
  • pancreas
  • rat

ASJC Scopus subject areas

  • Hepatology
  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Tariq, S., Rashed, H., Nurulain, S. M., Emerald, B. S., Koturan, S., Tekes, K., & Adeghate, E. (2015). Distribution of nociceptin in pancreatic islet cells of normal and diabetic rats. Pancreas., 44(4), 602-607. https://doi.org/10.1097/MPA.0000000000000306

Distribution of nociceptin in pancreatic islet cells of normal and diabetic rats. / Tariq, Saeed; Rashed, Hameed; Nurulain, Syed M.; Emerald, Bright Starling; Koturan, Surya; Tekes, K.; Adeghate, Ernest.

In: Pancreas., Vol. 44, No. 4, 25.05.2015, p. 602-607.

Research output: Contribution to journalArticle

Tariq, S, Rashed, H, Nurulain, SM, Emerald, BS, Koturan, S, Tekes, K & Adeghate, E 2015, 'Distribution of nociceptin in pancreatic islet cells of normal and diabetic rats', Pancreas., vol. 44, no. 4, pp. 602-607. https://doi.org/10.1097/MPA.0000000000000306
Tariq, Saeed ; Rashed, Hameed ; Nurulain, Syed M. ; Emerald, Bright Starling ; Koturan, Surya ; Tekes, K. ; Adeghate, Ernest. / Distribution of nociceptin in pancreatic islet cells of normal and diabetic rats. In: Pancreas. 2015 ; Vol. 44, No. 4. pp. 602-607.
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