Distribution of N-methyl-14C-labeled selegiline in the rat

Kornélia Tekes, Zita Pöstényi, Erzsébet B. Faigl, Kálmán Magyar, András Polyák, György Trencsényi, Lajos Balogh, Huba Kalász

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tissue distribution of selegiline including N-methyl-14C-selegiline was studied with three different techniques. Whole body autoradiography of labeled selegiline in rats completed the former results obtained in mice. Counting radioactivity by liquid scintillation method in various body compartments gave an in-depth numerical estimation of distribution, while RP-HPLC determination of selegiline determined the fate of intact, non-metabolized parent compound. Whole body autoradiography following 15 and 60min of intraperitoneal application of N-methyl-14C-selegiline verified definite and time-dependent blood-brain penetration of selegiline. Quantitative determination of tissue concentrations by liquid scintillation and RP-HPLC methods following 5, 15, 60 and 180min of intraperitoneal administration of selegiline unanimously verified both blood-brain and blood-testis penetration of the compound through the barrier.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume111
DOIs
Publication statusPublished - Jul 1 2015

Keywords

  • HPLC
  • Radiolabeled ligand
  • Rat
  • Selegiline
  • Whole body autoradiography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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