Distribution of immunoreactive dynorphin B in discrete areas of the rat brain and spinal cord

Nadav Zamir, M. Palkóvits, Eckard Weber, Michael J. Brownstein

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The distribution of immunoreactive (ir)-dynorphin B in 101 microdissected rat brain and spinal cord regions was determined using a specific radioimmunoassay. The highest concentration of dynorphin B in brain was found in the substantia nigra (1106.2 fmol/mg protein). Very high concentrations of ir-dynorphin B (> 400 fmol/mg protein) were also found in the lateral preoptic area, parabrachial nuclei and globus pallidus. Relatively high concentrations of ir-dynorphin B (250-400 fmol/mg protein) were found in 19 nuclei, including the periaqueductal gray matter, anterior hypothalamic nucleus, median eminence, nucleus accumbens and hippocampus. Moderate levels of the peptide (between 100 and 250 fmol/mg protein) were found in 42 brain nuclei such as the perifornical nucleus, nucleus of the diagonal band, medial forebrain bundle, and dorsal premamillary nucleus. Low concentrations of ir-dynorphin B (<100 fmol/mg protein) were found in 28 brain areas, e.g. cerebral cortical structures (parietal, cingulate, frontal), claustrum, olfactory bulb, lateral and periventricular thalamic nuclei. The cerebellar cortex has the lowest dynorphin B concentration (53.7 fmol/mg protein). Spinal cord segments exhibit low or moderate (cervical segment) levels of the peptide. The neurointermediate lobe of the pituitary gland is extremely rich in ir-dynorphin B (11,047.1 fmol/mg protein).

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalBrain Research
Volume300
Issue number1
DOIs
Publication statusPublished - May 21 1984

Fingerprint

Spinal Cord
Brain
Proteins
Anterior Hypothalamic Nucleus
Medial Forebrain Bundle
Lateral Thalamic Nuclei
Septal Nuclei
Periaqueductal Gray
Median Eminence
Peptides
Cerebellar Cortex
Globus Pallidus
Preoptic Area
Olfactory Bulb
Nucleus Accumbens
Substantia Nigra
Pituitary Gland
rimorphin
Basal Ganglia
Radioimmunoassay

Keywords

  • dynorphin B
  • Leu-enkephalin
  • posterior pituitary
  • rat brain nuclei
  • RIA
  • spinal cord

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Distribution of immunoreactive dynorphin B in discrete areas of the rat brain and spinal cord. / Zamir, Nadav; Palkóvits, M.; Weber, Eckard; Brownstein, Michael J.

In: Brain Research, Vol. 300, No. 1, 21.05.1984, p. 121-127.

Research output: Contribution to journalArticle

Zamir, Nadav ; Palkóvits, M. ; Weber, Eckard ; Brownstein, Michael J. / Distribution of immunoreactive dynorphin B in discrete areas of the rat brain and spinal cord. In: Brain Research. 1984 ; Vol. 300, No. 1. pp. 121-127.
@article{6b6c2004dd6b4edf8ce18a03f1e01f21,
title = "Distribution of immunoreactive dynorphin B in discrete areas of the rat brain and spinal cord",
abstract = "The distribution of immunoreactive (ir)-dynorphin B in 101 microdissected rat brain and spinal cord regions was determined using a specific radioimmunoassay. The highest concentration of dynorphin B in brain was found in the substantia nigra (1106.2 fmol/mg protein). Very high concentrations of ir-dynorphin B (> 400 fmol/mg protein) were also found in the lateral preoptic area, parabrachial nuclei and globus pallidus. Relatively high concentrations of ir-dynorphin B (250-400 fmol/mg protein) were found in 19 nuclei, including the periaqueductal gray matter, anterior hypothalamic nucleus, median eminence, nucleus accumbens and hippocampus. Moderate levels of the peptide (between 100 and 250 fmol/mg protein) were found in 42 brain nuclei such as the perifornical nucleus, nucleus of the diagonal band, medial forebrain bundle, and dorsal premamillary nucleus. Low concentrations of ir-dynorphin B (<100 fmol/mg protein) were found in 28 brain areas, e.g. cerebral cortical structures (parietal, cingulate, frontal), claustrum, olfactory bulb, lateral and periventricular thalamic nuclei. The cerebellar cortex has the lowest dynorphin B concentration (53.7 fmol/mg protein). Spinal cord segments exhibit low or moderate (cervical segment) levels of the peptide. The neurointermediate lobe of the pituitary gland is extremely rich in ir-dynorphin B (11,047.1 fmol/mg protein).",
keywords = "dynorphin B, Leu-enkephalin, posterior pituitary, rat brain nuclei, RIA, spinal cord",
author = "Nadav Zamir and M. Palk{\'o}vits and Eckard Weber and Brownstein, {Michael J.}",
year = "1984",
month = "5",
day = "21",
doi = "10.1016/0006-8993(84)91346-5",
language = "English",
volume = "300",
pages = "121--127",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Distribution of immunoreactive dynorphin B in discrete areas of the rat brain and spinal cord

AU - Zamir, Nadav

AU - Palkóvits, M.

AU - Weber, Eckard

AU - Brownstein, Michael J.

PY - 1984/5/21

Y1 - 1984/5/21

N2 - The distribution of immunoreactive (ir)-dynorphin B in 101 microdissected rat brain and spinal cord regions was determined using a specific radioimmunoassay. The highest concentration of dynorphin B in brain was found in the substantia nigra (1106.2 fmol/mg protein). Very high concentrations of ir-dynorphin B (> 400 fmol/mg protein) were also found in the lateral preoptic area, parabrachial nuclei and globus pallidus. Relatively high concentrations of ir-dynorphin B (250-400 fmol/mg protein) were found in 19 nuclei, including the periaqueductal gray matter, anterior hypothalamic nucleus, median eminence, nucleus accumbens and hippocampus. Moderate levels of the peptide (between 100 and 250 fmol/mg protein) were found in 42 brain nuclei such as the perifornical nucleus, nucleus of the diagonal band, medial forebrain bundle, and dorsal premamillary nucleus. Low concentrations of ir-dynorphin B (<100 fmol/mg protein) were found in 28 brain areas, e.g. cerebral cortical structures (parietal, cingulate, frontal), claustrum, olfactory bulb, lateral and periventricular thalamic nuclei. The cerebellar cortex has the lowest dynorphin B concentration (53.7 fmol/mg protein). Spinal cord segments exhibit low or moderate (cervical segment) levels of the peptide. The neurointermediate lobe of the pituitary gland is extremely rich in ir-dynorphin B (11,047.1 fmol/mg protein).

AB - The distribution of immunoreactive (ir)-dynorphin B in 101 microdissected rat brain and spinal cord regions was determined using a specific radioimmunoassay. The highest concentration of dynorphin B in brain was found in the substantia nigra (1106.2 fmol/mg protein). Very high concentrations of ir-dynorphin B (> 400 fmol/mg protein) were also found in the lateral preoptic area, parabrachial nuclei and globus pallidus. Relatively high concentrations of ir-dynorphin B (250-400 fmol/mg protein) were found in 19 nuclei, including the periaqueductal gray matter, anterior hypothalamic nucleus, median eminence, nucleus accumbens and hippocampus. Moderate levels of the peptide (between 100 and 250 fmol/mg protein) were found in 42 brain nuclei such as the perifornical nucleus, nucleus of the diagonal band, medial forebrain bundle, and dorsal premamillary nucleus. Low concentrations of ir-dynorphin B (<100 fmol/mg protein) were found in 28 brain areas, e.g. cerebral cortical structures (parietal, cingulate, frontal), claustrum, olfactory bulb, lateral and periventricular thalamic nuclei. The cerebellar cortex has the lowest dynorphin B concentration (53.7 fmol/mg protein). Spinal cord segments exhibit low or moderate (cervical segment) levels of the peptide. The neurointermediate lobe of the pituitary gland is extremely rich in ir-dynorphin B (11,047.1 fmol/mg protein).

KW - dynorphin B

KW - Leu-enkephalin

KW - posterior pituitary

KW - rat brain nuclei

KW - RIA

KW - spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0021266840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021266840&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(84)91346-5

DO - 10.1016/0006-8993(84)91346-5

M3 - Article

C2 - 6733459

AN - SCOPUS:0021266840

VL - 300

SP - 121

EP - 127

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -