Distribution of immunoreactive β-neo-endorphin in discrete areas of the rat brain and pituitary gland: Comparison with α-neo-endorphin

N. Zamir, M. Palkovits, M. J. Brownstein

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The distribution of immunoreactive (ir)-β-neo-endorphin in 101 microdissected rat brain and spinal cord regions as well as in the neurointermediate lobe of pituitary gland was determined using a highly specific radioimmunoassay. The highest concentration of β-neo-endorphin in brain was found in the median eminence (341.4 fmol/mg of protein). High concentrations of ir-β-neo-endorphin (>250 fmol/mg of protein) were found in 11 nuclei, including dorsomedial nucleus, substantia nigra, parabrachial nuclei, periaqueductal gray matter, anterior hypothalamic nucleus, and lateral preoptic areas. Moderate concentrations of the peptide (between 100 and 250 fmol/mg of protein) were found in 66 brain nuclei such as the amygdaloid and septal nuclei, most of the diencephalic structures (not including the hypothalamus), and the majority of the medulla oblongata nuclei and others. Low concentrations of ir-β-neo-endorphin (<100 fmol/mg of protein) were found in 21 nuclei, e.g., cortical structures (frontal, dingulate, piriform, parietal, entorhinal, occipital), olfactory tubercle, and cerebellum (nuclei and cortex). The olfactory bulb has the lowest β-neo-endorphin concentration (21.3 fmol/mg of protein). Spinal cord segments exhibit low peptide concentrations. The neurointermediate lobe of the pituitary gland is extremely rich in ir-β-neo-endorphin.

Original languageEnglish
Pages (from-to)1248-1252
Number of pages5
JournalJournal of Neuroscience
Volume4
Issue number5
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Neuroscience(all)

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