Distribution of galanin‐like immunoreactivity in the brain of Rana esculenta and Xenopus laevis

Gyula Y. Lázár, Zsolt S. Liposits, Pál Tóth, Scott L. Trasti, Jerome L. Maderdrut, István Merchenthaler

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Abstract

The immunocytochemical distribution of galanin‐containing perikarya and nerve terminals in the brain of Rana esculenta and Xenopus laevis was determined with antisera directed toward either porcine or rat galanin. The pattern of galanin‐like immunoreactivity appeared to be identical with antisera directed toward either target antige. The distribution of galanin‐like immunoreactivity was similar in Rana esculenta and Xenopus laevis except for the absence of a distinct laminar distribution of immunoreactivity in the optic tectum of Xenopus laevis. Galanin‐containing perikarya were located in all major subdivisions of the brain except the metencephalon. In the telencephalon, immunoreactive perikarya were detected in the pars medialis of the amygdala and the preoptic area. In the diencephalon, immunoreactive perikarya were detected in the caudal half of the suprachiasmatic nucleus, the nucleus of the periventricular organ, the ventral hypothalamus, and the median eminence. In the mesencephalon, immunoreactive perikarya were detected near the midline of the rostroventral tegmentum, in the torus semicircularis and, occasionally, in lamina A and layer 6 of the optic tectum. In the myelencephalon, labelled perikarya were detected only in the caudal half of the nucleus of the solitary tract. Immunoreactive nerve fibers of varying density were observed in all subdivisions of the brain with the densest accumulations of fibers occurring in the pars lateralis of the amygdala and the preoptic area. Dense accumulations of nerve fibers were also found in the lateral septum, the medial forebrain bundle, the periventricular region of the diencephalon, the ventral hypothalamus, the median eminence, the mesencephalic central gray, the laminar nucleus of the torus semicircularis, several laminae of the optic tectum, the interpeduncular nucleus, the isthmic nucleus, the central gray of the rhombencephalon, and the dorsolateral caudal medull. The extensive system of galanin‐containing perikarya and nerve fibers in the brain of representatives of two families of anurans showed many similarities to the distribution of galanin‐containing perikarya and nerve fibers previously described for the mammalian brai.

Original languageEnglish
Pages (from-to)45-67
Number of pages23
JournalJournal of Comparative Neurology
Volume310
Issue number1
DOIs
Publication statusPublished - Aug 1 1991

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Keywords

  • evolution
  • frog
  • immunocytochemistry
  • neuroanatomy
  • neuroendocrinology
  • neuropeptides
  • optic tectum
  • vision

ASJC Scopus subject areas

  • Neuroscience(all)

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