Cholecystokinin (CCK) concentrations were determined in microdissected individual nuclei of the hypothalamus and limbic system using a specific and sensitive CCK radioimmunoassay (RIA). The CCK levels in the hypothalamus were highest (> 2 mg CCK8 sulfate equivalents/mg protein) in the ventromedial, dorsomedial, periventricular, arcuate nuclei, the median eminence, and mamillary body. Most regions of the limbic system had higher levels of CCK than the hypothalamus and in particular the CA3 region of the hippocampus, the lateral septal nucleus and the medial and lateral amygdaloid nuclei had levels of CCK which were similar to some cerebral cortical areas. This study confirms previous preliminary CCK distribution studies in the rat and indicates that CCK in addition to its wide distribution in the cerebral cortex, is present in abundance in many areas of the hypothalamus and limbic system.
ASJC Scopus subject areas
- Endocrine and Autonomic Systems
- Cellular and Molecular Neuroscience