Distribution of catch-relaxing peptide (CARP)-like immunoreactive neurons in the central and peripheral nervous system of Helix pomatia

L. Hernádi, Y. Terano, Y. Muneoka, T. Kiss

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Immunocytochemistry was performed on the nervous system of Helix by the use of an antibody raised against a myotropic neuropeptide, the catch-relaxing peptide (CARP), isolated from Mytilus edulis. In each ganglion of the central nervous system of Helix pomatia, numerous CARP-immunoreactive cell bodies and a dense immunoreactive fiber system could be observed with a dominancy in the cerebral and pedal ganglia. The majority of the immunoreactive neurons are unipolar, although multipolar neurons also occur. In the neuropil areas, CARP-immunoreactive fibers show extensive arborization, which may indicate a central role of CARP. CARP-immunoreactive elements could be observed in each investigated peripheral nerve and peripheral areas, namely in the intestine, heart, aorta, buccal mass, lips, and foot. However, CARP-immunoreactive cell bodies could only be demonstrated in the intestine and the foot musculature. Thin varicose CARP-immunoreactive fibers were observed over both muscle and gland cells in the different peripheral organs, suggesting a peripheral role of CARP. In vivo CARP injection into the body cavity (10-3, 10-4, 10-5 M) altered the general behavioral state of the animals and induced the relaxation of the musculature of the whole body wall indicating that CARP has a significant role in the regulation of muscle contraction.

Original languageEnglish
Pages (from-to)335-348
Number of pages14
JournalCell and Tissue Research
Volume280
Issue number2
DOIs
Publication statusPublished - May 1995

Fingerprint

Peripheral Nervous System
Neurology
Neurons
Central Nervous System
Peptides
Foot
Ganglia
Intestines
Muscle
Fibers
Mytilus edulis
Cells
Neuropil
Cheek
Muscle Contraction
Lip
Neuropeptides
Peripheral Nerves
Muscle Cells
Nervous System

Keywords

  • Catch-relaxing peptide (CARP)
  • Helix pomatia (Mollusca)
  • Immunocytochemistry
  • Nervous system, central
  • Nervous system, peripheral

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Distribution of catch-relaxing peptide (CARP)-like immunoreactive neurons in the central and peripheral nervous system of Helix pomatia. / Hernádi, L.; Terano, Y.; Muneoka, Y.; Kiss, T.

In: Cell and Tissue Research, Vol. 280, No. 2, 05.1995, p. 335-348.

Research output: Contribution to journalArticle

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