Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas

B. Burghardt, M. L. Elkjær, T. H. Kwon, G. Z. Rácz, G. Varga, M. C. Steward, S. Nielsen

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Background: The exocrine pancreas secretes large volumes of isotonic fluid, most of which originates from the ductal system. The role of aquaporin (AQP) water channels in this process is unknown. Methods: Expression and localisation of known AQP isoforms was examined in normal human pancreas, pancreatic adenocarcinoma, and pancreatic cell lines of ductal origin (Capan-1, Capan-2, and HPAF) using reverse transcriptase-polymerase chain reaction and immunohistochemistry. Results: Messenger RNAs for AQP1, -3, -4, -5, and -8 were detected in normal pancreas and in pancreatic adenocarcinoma. The cell lines expressed AQP3, -4, and -5 but lacked AQP1 and AQP8. Immunohistochemistry of normal pancreas revealed that AQP1 is strongly expressed in centroacinar cells and in both the apical and basolateral domains of intercalated and intralobular duct epithelia. AQP1 expression declined with distance along the small interlobular ducts and was not detectable in larger intedobular ducts. AQP3 and AQP4 were not detectable by immunohistochemistry. AQP5 was observed at the apical membrane of intercalated duct cells and also in duct associated mucoid glands. AQP8 was confined to the apical pole of acinar cells. Both AQP1 and AQP5 were colocalised with cystic fibrosis transmembrane conductance regulator (CFTR) at the apical membrane of intercalated duct cells. Conclusions: AQP1 and AQP5 are strongly expressed in the intercalated ducts of the human pancreas. Their distribution correlates closely with that of CFTR, a marker of ductal electrolyte secretion. This suggests that fluid secretion is concentrated in the terminal branches of the ductal tree and that both AQP1 and AQP5 may play a significant role.

Original languageEnglish
Pages (from-to)1008-1016
Number of pages9
JournalGut
Volume52
Issue number7
DOIs
Publication statusPublished - Jul 1 2003

Fingerprint

Aquaporins
Pancreas
Cystic Fibrosis Transmembrane Conductance Regulator
Immunohistochemistry
Adenocarcinoma
Fluids and Secretions
Cell Line
Exocrine Pancreas
Membranes
Acinar Cells
Reverse Transcriptase Polymerase Chain Reaction
Electrolytes
Protein Isoforms
Epithelium
Messenger RNA

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas. / Burghardt, B.; Elkjær, M. L.; Kwon, T. H.; Rácz, G. Z.; Varga, G.; Steward, M. C.; Nielsen, S.

In: Gut, Vol. 52, No. 7, 01.07.2003, p. 1008-1016.

Research output: Contribution to journalArticle

Burghardt, B. ; Elkjær, M. L. ; Kwon, T. H. ; Rácz, G. Z. ; Varga, G. ; Steward, M. C. ; Nielsen, S. / Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas. In: Gut. 2003 ; Vol. 52, No. 7. pp. 1008-1016.
@article{4876918851aa4e0ca5a4cffb03c5df0f,
title = "Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas",
abstract = "Background: The exocrine pancreas secretes large volumes of isotonic fluid, most of which originates from the ductal system. The role of aquaporin (AQP) water channels in this process is unknown. Methods: Expression and localisation of known AQP isoforms was examined in normal human pancreas, pancreatic adenocarcinoma, and pancreatic cell lines of ductal origin (Capan-1, Capan-2, and HPAF) using reverse transcriptase-polymerase chain reaction and immunohistochemistry. Results: Messenger RNAs for AQP1, -3, -4, -5, and -8 were detected in normal pancreas and in pancreatic adenocarcinoma. The cell lines expressed AQP3, -4, and -5 but lacked AQP1 and AQP8. Immunohistochemistry of normal pancreas revealed that AQP1 is strongly expressed in centroacinar cells and in both the apical and basolateral domains of intercalated and intralobular duct epithelia. AQP1 expression declined with distance along the small interlobular ducts and was not detectable in larger intedobular ducts. AQP3 and AQP4 were not detectable by immunohistochemistry. AQP5 was observed at the apical membrane of intercalated duct cells and also in duct associated mucoid glands. AQP8 was confined to the apical pole of acinar cells. Both AQP1 and AQP5 were colocalised with cystic fibrosis transmembrane conductance regulator (CFTR) at the apical membrane of intercalated duct cells. Conclusions: AQP1 and AQP5 are strongly expressed in the intercalated ducts of the human pancreas. Their distribution correlates closely with that of CFTR, a marker of ductal electrolyte secretion. This suggests that fluid secretion is concentrated in the terminal branches of the ductal tree and that both AQP1 and AQP5 may play a significant role.",
author = "B. Burghardt and Elkj{\ae}r, {M. L.} and Kwon, {T. H.} and R{\'a}cz, {G. Z.} and G. Varga and Steward, {M. C.} and S. Nielsen",
year = "2003",
month = "7",
day = "1",
doi = "10.1136/gut.52.7.1008",
language = "English",
volume = "52",
pages = "1008--1016",
journal = "Gut",
issn = "0017-5749",
publisher = "BMJ Publishing Group",
number = "7",

}

TY - JOUR

T1 - Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas

AU - Burghardt, B.

AU - Elkjær, M. L.

AU - Kwon, T. H.

AU - Rácz, G. Z.

AU - Varga, G.

AU - Steward, M. C.

AU - Nielsen, S.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Background: The exocrine pancreas secretes large volumes of isotonic fluid, most of which originates from the ductal system. The role of aquaporin (AQP) water channels in this process is unknown. Methods: Expression and localisation of known AQP isoforms was examined in normal human pancreas, pancreatic adenocarcinoma, and pancreatic cell lines of ductal origin (Capan-1, Capan-2, and HPAF) using reverse transcriptase-polymerase chain reaction and immunohistochemistry. Results: Messenger RNAs for AQP1, -3, -4, -5, and -8 were detected in normal pancreas and in pancreatic adenocarcinoma. The cell lines expressed AQP3, -4, and -5 but lacked AQP1 and AQP8. Immunohistochemistry of normal pancreas revealed that AQP1 is strongly expressed in centroacinar cells and in both the apical and basolateral domains of intercalated and intralobular duct epithelia. AQP1 expression declined with distance along the small interlobular ducts and was not detectable in larger intedobular ducts. AQP3 and AQP4 were not detectable by immunohistochemistry. AQP5 was observed at the apical membrane of intercalated duct cells and also in duct associated mucoid glands. AQP8 was confined to the apical pole of acinar cells. Both AQP1 and AQP5 were colocalised with cystic fibrosis transmembrane conductance regulator (CFTR) at the apical membrane of intercalated duct cells. Conclusions: AQP1 and AQP5 are strongly expressed in the intercalated ducts of the human pancreas. Their distribution correlates closely with that of CFTR, a marker of ductal electrolyte secretion. This suggests that fluid secretion is concentrated in the terminal branches of the ductal tree and that both AQP1 and AQP5 may play a significant role.

AB - Background: The exocrine pancreas secretes large volumes of isotonic fluid, most of which originates from the ductal system. The role of aquaporin (AQP) water channels in this process is unknown. Methods: Expression and localisation of known AQP isoforms was examined in normal human pancreas, pancreatic adenocarcinoma, and pancreatic cell lines of ductal origin (Capan-1, Capan-2, and HPAF) using reverse transcriptase-polymerase chain reaction and immunohistochemistry. Results: Messenger RNAs for AQP1, -3, -4, -5, and -8 were detected in normal pancreas and in pancreatic adenocarcinoma. The cell lines expressed AQP3, -4, and -5 but lacked AQP1 and AQP8. Immunohistochemistry of normal pancreas revealed that AQP1 is strongly expressed in centroacinar cells and in both the apical and basolateral domains of intercalated and intralobular duct epithelia. AQP1 expression declined with distance along the small interlobular ducts and was not detectable in larger intedobular ducts. AQP3 and AQP4 were not detectable by immunohistochemistry. AQP5 was observed at the apical membrane of intercalated duct cells and also in duct associated mucoid glands. AQP8 was confined to the apical pole of acinar cells. Both AQP1 and AQP5 were colocalised with cystic fibrosis transmembrane conductance regulator (CFTR) at the apical membrane of intercalated duct cells. Conclusions: AQP1 and AQP5 are strongly expressed in the intercalated ducts of the human pancreas. Their distribution correlates closely with that of CFTR, a marker of ductal electrolyte secretion. This suggests that fluid secretion is concentrated in the terminal branches of the ductal tree and that both AQP1 and AQP5 may play a significant role.

UR - http://www.scopus.com/inward/record.url?scp=0038460939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038460939&partnerID=8YFLogxK

U2 - 10.1136/gut.52.7.1008

DO - 10.1136/gut.52.7.1008

M3 - Article

C2 - 12801959

AN - SCOPUS:0038460939

VL - 52

SP - 1008

EP - 1016

JO - Gut

JF - Gut

SN - 0017-5749

IS - 7

ER -