1. 1. Defined fractions of nervous tissue from Anodonta were obtained by a combination of differential and gradient centrifugation and analysed for their amines and amino acids by using a sensitive micro dansyl procedure. 2. 2. Of the 17 substances analysed, serotonin and tryptophan were particularly associated with fractions containing synaptic endings and dense-core vesicles. No other substance was selectively associated with these fractions. 3. 3. Of the amino acids, ornithine, lysine, leucine, isoleucine, valine, GABA, alanine, methionine, aspartic and glutamic acids had a similar distribution in the different primary fractions and subfractions of the mitochondrial (P2) fractions. In contrast proline and glycine were particularly associated with the supernatant (S) fraction and phenylalanine in addition to tryptophan with the mitochondrial (P2) fraction. 4. 4. High levels of putrescine also occur in the nervous system and the amine was particularly associated with the supernatant (S) and microsomal (P3) fractions. 5. 5. The significance of these results is discussed.
|Number of pages||5|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Publication status||Published - 1976|
ASJC Scopus subject areas
- Molecular Biology