Distribution of β-dystroglycan immunopositive globules in the subventricular zone of rat brain

Istvan Adorjan, M. Kálmán

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Present study demonstrates a novel localization of β-dystroglycan in rat brain. Beside the meningeal surface and the cerebral vessels where β-dystroglycan immunopositivity has been described, now immunopositive solid bodies were found at the basis of ependymocytes. Since they proved to be round in either coronal, sagittal, and horizontal section, revealing their globular shape, they received the term 'aglobules.' Double immunofluorescent labeling of GFAP (glial fibrillary acidic protein) and β-dystroglycan revealed that 'aglobules' are within the 'cordon' of subventricular astrocytes. The 'aglobules' were found throughout the ventricular system, except the ventral part and floor of the third ventricle. The latter one comprised the median eminence and extended caudally to the inframamillary recess. The area where the 'aglobules' were missing, corresponded to that where ependymocytes were replaced by GFAP-immunopositive tanycytes. Utrophin and α-dystrobrevin were colocalized with dystroglycan, whereas α1-syntrophin was detected along the borders of the ependymal cells. Comparing our results to former data, the 'aglobules' seem to be the anchoring places of the afractons' formed by laminin [Mercier et al. (2003) J Comp Neurol 455:324-340], which interconnect the vascular basal lamina and the ependymal layer. The ependymal localization of the 'aglobules' was proved by pre-embedding electron microscopic immunohistochemistry. The 'aglobules' proved to be a labyrinthine structure, which seemed to be identical with the 'basal membrane labyrinth' described by Leonhardt [Leonhardt (1970) Z Zellforsch Mikrosk Anat 105:395-404]. The description of the β-dystroglycan-immunopositive 'aglobules' contributes to the better understanding of the structure of the subventricular zone where neurogenesis can occur during adulthood and provides an important link to the meningeo-glial network.

Original languageEnglish
Pages (from-to)657-666
Number of pages10
JournalGLIA
Volume57
Issue number6
DOIs
Publication statusPublished - Apr 15 2009

Fingerprint

Dystroglycans
Lateral Ventricles
Brain
Glial Fibrillary Acidic Protein
Utrophin
Ependymoglial Cells
Median Eminence
Third Ventricle
Neurogenesis
Laminin
Inner Ear
Basement Membrane
Neuroglia
Astrocytes
Blood Vessels
Immunohistochemistry
Electrons
Membranes

Keywords

  • Basal membrane labyrinth
  • Dystrobrevin
  • Ependyma
  • Tanycyte
  • Utrophin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Distribution of β-dystroglycan immunopositive globules in the subventricular zone of rat brain. / Adorjan, Istvan; Kálmán, M.

In: GLIA, Vol. 57, No. 6, 15.04.2009, p. 657-666.

Research output: Contribution to journalArticle

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