Distribution and projections of cholecystokinin-immunoreactive neurons in the hypothalamic paraventricular nucleus of rat

J. Kiss, T. H. Williams, M. Palkóvits

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Analysis of coronal sections from colchicine-treated rat brains reveals that CCK-immunoreactivity (CCK-ir) is present in two distinguishable neuronal systems in the paraventricular nucleus (PVN). More than 60% of these cells were found to be typical parvicellular neurons; the remainder were magnocellular neurons. The magnocellular CCK-ir neurons were concentrated in the medial magnocellular subdivision, while more caudally they formed a ring around a zone of unstained magnocellular neurons. Immunostained parvicellular neurons predominate in medial and periventricular parvicellular subdivisions. The efferent projections of CCK-ir neurons were investigated by looking for retrograde accumulation of CCK-ir in cell bodies after selective knife cuts. A parasagittal cut of the lateral retrochiasmatic area as well as transection of the rostral median eminence resulted in an accumulation of CCK-ir material in a large number of both parvi- and magnocellular neurons. After pituitary stalk lesions, however, increased staining was only seen in magnocellular neurons. It is inferred that the magnocellular (presumed oxytocin-CCK) cells send their axons to the pituitary, whereas axons of CCK-ir parvicellular neurons appear to terminate in the median eminence. After transection of the medial forebrain bundle (MFB), immunostaining increased in a small number of scattered transected fibers proximal to the knife cut and in a few perikarya in the PVN, indicating that very few CCK cells may send descending fibers to the lower brainstem.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalJournal of Comparative Neurology
Volume227
Issue number2
Publication statusPublished - 1984

Fingerprint

Paraventricular Hypothalamic Nucleus
Cholecystokinin
Neurons
Median Eminence
Axons
Medial Forebrain Bundle
Colchicine
Pituitary Gland
Oxytocin
Brain Stem
Staining and Labeling
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{2fd4cb0e9241412eae8d9c113826b229,
title = "Distribution and projections of cholecystokinin-immunoreactive neurons in the hypothalamic paraventricular nucleus of rat",
abstract = "Analysis of coronal sections from colchicine-treated rat brains reveals that CCK-immunoreactivity (CCK-ir) is present in two distinguishable neuronal systems in the paraventricular nucleus (PVN). More than 60{\%} of these cells were found to be typical parvicellular neurons; the remainder were magnocellular neurons. The magnocellular CCK-ir neurons were concentrated in the medial magnocellular subdivision, while more caudally they formed a ring around a zone of unstained magnocellular neurons. Immunostained parvicellular neurons predominate in medial and periventricular parvicellular subdivisions. The efferent projections of CCK-ir neurons were investigated by looking for retrograde accumulation of CCK-ir in cell bodies after selective knife cuts. A parasagittal cut of the lateral retrochiasmatic area as well as transection of the rostral median eminence resulted in an accumulation of CCK-ir material in a large number of both parvi- and magnocellular neurons. After pituitary stalk lesions, however, increased staining was only seen in magnocellular neurons. It is inferred that the magnocellular (presumed oxytocin-CCK) cells send their axons to the pituitary, whereas axons of CCK-ir parvicellular neurons appear to terminate in the median eminence. After transection of the medial forebrain bundle (MFB), immunostaining increased in a small number of scattered transected fibers proximal to the knife cut and in a few perikarya in the PVN, indicating that very few CCK cells may send descending fibers to the lower brainstem.",
author = "J. Kiss and Williams, {T. H.} and M. Palk{\'o}vits",
year = "1984",
language = "English",
volume = "227",
pages = "173--181",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Distribution and projections of cholecystokinin-immunoreactive neurons in the hypothalamic paraventricular nucleus of rat

AU - Kiss, J.

AU - Williams, T. H.

AU - Palkóvits, M.

PY - 1984

Y1 - 1984

N2 - Analysis of coronal sections from colchicine-treated rat brains reveals that CCK-immunoreactivity (CCK-ir) is present in two distinguishable neuronal systems in the paraventricular nucleus (PVN). More than 60% of these cells were found to be typical parvicellular neurons; the remainder were magnocellular neurons. The magnocellular CCK-ir neurons were concentrated in the medial magnocellular subdivision, while more caudally they formed a ring around a zone of unstained magnocellular neurons. Immunostained parvicellular neurons predominate in medial and periventricular parvicellular subdivisions. The efferent projections of CCK-ir neurons were investigated by looking for retrograde accumulation of CCK-ir in cell bodies after selective knife cuts. A parasagittal cut of the lateral retrochiasmatic area as well as transection of the rostral median eminence resulted in an accumulation of CCK-ir material in a large number of both parvi- and magnocellular neurons. After pituitary stalk lesions, however, increased staining was only seen in magnocellular neurons. It is inferred that the magnocellular (presumed oxytocin-CCK) cells send their axons to the pituitary, whereas axons of CCK-ir parvicellular neurons appear to terminate in the median eminence. After transection of the medial forebrain bundle (MFB), immunostaining increased in a small number of scattered transected fibers proximal to the knife cut and in a few perikarya in the PVN, indicating that very few CCK cells may send descending fibers to the lower brainstem.

AB - Analysis of coronal sections from colchicine-treated rat brains reveals that CCK-immunoreactivity (CCK-ir) is present in two distinguishable neuronal systems in the paraventricular nucleus (PVN). More than 60% of these cells were found to be typical parvicellular neurons; the remainder were magnocellular neurons. The magnocellular CCK-ir neurons were concentrated in the medial magnocellular subdivision, while more caudally they formed a ring around a zone of unstained magnocellular neurons. Immunostained parvicellular neurons predominate in medial and periventricular parvicellular subdivisions. The efferent projections of CCK-ir neurons were investigated by looking for retrograde accumulation of CCK-ir in cell bodies after selective knife cuts. A parasagittal cut of the lateral retrochiasmatic area as well as transection of the rostral median eminence resulted in an accumulation of CCK-ir material in a large number of both parvi- and magnocellular neurons. After pituitary stalk lesions, however, increased staining was only seen in magnocellular neurons. It is inferred that the magnocellular (presumed oxytocin-CCK) cells send their axons to the pituitary, whereas axons of CCK-ir parvicellular neurons appear to terminate in the median eminence. After transection of the medial forebrain bundle (MFB), immunostaining increased in a small number of scattered transected fibers proximal to the knife cut and in a few perikarya in the PVN, indicating that very few CCK cells may send descending fibers to the lower brainstem.

UR - http://www.scopus.com/inward/record.url?scp=0021171857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021171857&partnerID=8YFLogxK

M3 - Article

C2 - 6381557

AN - SCOPUS:0021171857

VL - 227

SP - 173

EP - 181

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 2

ER -