Distribution and orientation of hydrogenase in various photosynthetic bacteria

Kornel L. Kovacs, C. Bagyinka, Larissa T. Serebriakova

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

All of the tested photosynthetic bacteria possessed hydrogenase activity. Cell membranes of representatives of the Chromatiaceae, Rhodospirillaceae, and Chlorobiaceae families were found to be impermeable to the oxidized redox dyes methyl viologen and benzyl viologen, whereas the reduced forms could easily penetrate the membranes. This permeability difference made possible the localization of the hydrogenase enzyme. Members of the Chromatiaceae and the Rhodospirillaceae contained a predominantly or exclusively membranebound enzyme. In contrast, the majority of hydrogenase activity was in the cytoplasm of Chlorobium limicola f. thiosulfatophilum. Hydrogenase, or at least its active center, was oriented toward the outer surface of the cell membrane in purple bacteria.

Original languageEnglish
Pages (from-to)215-218
Number of pages4
JournalCurrent Microbiology
Volume9
Issue number4
DOIs
Publication statusPublished - Jul 1 1983

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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