Distortion of the lamellar arrangement of phospholipids by deep rough mutant lipopolysaccharide from Salmonella minnesota

Edit Urbán, A. Bóta, B. Kocsis, K. Lohner

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The concentration dependent effects of deep rough mutant lipopolysaccharide (LPS) from Salmonella minnesota (R595) on two different phospholipid model membranes was investigated by differential scanning calorimetry and small-angle X-ray scattering (SAXS). At low concentrations of LPS the well ordered multilamellar arrangement of dipalmitoylphosphatidylcholine (DPPC) vesicles is strongly distorted resulting in a loss of positional correlation of the lipid lamellae and smaller domain sizes within the lamellae. The pre-transition of DPPC was abolished at a LPS/DPPC molar ratio of 0.1:1 and the main or chain melting transition was strongly broadened. Moreover, the enthalpy was significantly decreased and a transition was hardly detected at an equimolar mixture of LPS/DPPC. LPS also affected the lamellar arrangement of a mixture of dipalmitoylphosphatidylethanolamine (DPPE) and dipalmitoylphosphatidylglycerol (DPPG). Furthermore, a phase separation was observed for this phospholipid mixture resulting in DPPE enriched and depleted domains. Similarly to DPPC, only a weak phase transition was observed at the highest LPS concentration used (LPS/DPPE-DPPG 1:1 mol/mol). SAXS measurements showed that for both systems increasing the concentration of LPS resulted in a concomitant increase of the formation of cubic structures, which are predominant at an equimolar mixture of LPS/phospholipid. However, because of the small number of peaks it was not possible to unambiguously identify the space group of the cubic structure, complicated by the coexistence with a lamellar phase, which was particularly detectable for the LPS/DPPC mixture.

Original languageEnglish
Pages (from-to)463-469
Number of pages7
JournalJournal of Thermal Analysis and Calorimetry
Volume82
Issue number2
DOIs
Publication statusPublished - Nov 2005

Fingerprint

salmonella
Salmonella
Phospholipids
Lipopolysaccharides
1,2-Dipalmitoylphosphatidylcholine
lamella
X ray scattering
scattering
Phase separation
Lipids
lipids
low concentrations
Differential scanning calorimetry
Enthalpy
Melting
x rays
heat measurement
Phase transitions
enthalpy
melting

Keywords

  • Calorimetry
  • Cubic structures
  • Lipid mixtures
  • Phosphatidylcholine
  • Phosphatidylethanolamine
  • Phosphatidylglycerol
  • X-ray scattering

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Distortion of the lamellar arrangement of phospholipids by deep rough mutant lipopolysaccharide from Salmonella minnesota. / Urbán, Edit; Bóta, A.; Kocsis, B.; Lohner, K.

In: Journal of Thermal Analysis and Calorimetry, Vol. 82, No. 2, 11.2005, p. 463-469.

Research output: Contribution to journalArticle

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AB - The concentration dependent effects of deep rough mutant lipopolysaccharide (LPS) from Salmonella minnesota (R595) on two different phospholipid model membranes was investigated by differential scanning calorimetry and small-angle X-ray scattering (SAXS). At low concentrations of LPS the well ordered multilamellar arrangement of dipalmitoylphosphatidylcholine (DPPC) vesicles is strongly distorted resulting in a loss of positional correlation of the lipid lamellae and smaller domain sizes within the lamellae. The pre-transition of DPPC was abolished at a LPS/DPPC molar ratio of 0.1:1 and the main or chain melting transition was strongly broadened. Moreover, the enthalpy was significantly decreased and a transition was hardly detected at an equimolar mixture of LPS/DPPC. LPS also affected the lamellar arrangement of a mixture of dipalmitoylphosphatidylethanolamine (DPPE) and dipalmitoylphosphatidylglycerol (DPPG). Furthermore, a phase separation was observed for this phospholipid mixture resulting in DPPE enriched and depleted domains. Similarly to DPPC, only a weak phase transition was observed at the highest LPS concentration used (LPS/DPPE-DPPG 1:1 mol/mol). SAXS measurements showed that for both systems increasing the concentration of LPS resulted in a concomitant increase of the formation of cubic structures, which are predominant at an equimolar mixture of LPS/phospholipid. However, because of the small number of peaks it was not possible to unambiguously identify the space group of the cubic structure, complicated by the coexistence with a lamellar phase, which was particularly detectable for the LPS/DPPC mixture.

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