Distinctive role of IgG1 and IgG3 isotypes in FcγR-mediated functions

Z. Rozsnyay, G. Sarmay, M. Walker, K. Maslanka, Z. Valasek, R. Jefferis, J. Gergely

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Abstract

Polyclonal and monoclonal anti-Rh (D) antibodies of IgG1 and IgG3 subclass were evaluated for their capacity to sensitize erythrocytes and (i) to trigger monocyte and K-cell mediated antibody-dependent cellular cytotoxicity (ADCC); (ii) to mediate binding to monocyte and lymphocyte FcγR; (iii) to stimulate phagocytosis by monocytes. All antibodies were equally effective in mediating monocyte or activated U937 cell ADCC but IgG1 was more active than IgG3 in K-cell mediated ADCC. IgG3-sensitized erythrocytes inhibited IgG1-induced lysis, suggesting that each subclass engages the same FcγR receptor but that lysis requires a further 'signal' that the IgG3 molecule can not deliver. Two monoclonal IgG3 anti-D antibodies were shown to have higher binding (two times) and phagocytic (three times) indices than IgG1 antibody for monocytes; similar differences were observed for polyclonal IgG1 and IgG3 antibodies. The same pattern was observed in an EA rosette assay when a total lymphocyte population was used; however, this difference was not seen with a B-cell depleted (T+ null cell) lymphocyte population.

Original languageEnglish
Pages (from-to)491-498
Number of pages8
JournalImmunology
Volume66
Issue number4
Publication statusPublished - Jan 1 1989

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Rozsnyay, Z., Sarmay, G., Walker, M., Maslanka, K., Valasek, Z., Jefferis, R., & Gergely, J. (1989). Distinctive role of IgG1 and IgG3 isotypes in FcγR-mediated functions. Immunology, 66(4), 491-498.