Distinct subsets of nucleus basalis neurons exhibit similar sensitivity to excitotoxicity

Tibor Harkany, Csaba Varga, Jens Grosche, Jan Mulder, Paul G M Luiten, Tibor Hortobágyi, Botond Penke, Wolfgang Härtig

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Excitotoxic lesions in the magnocellular nucleus basalis (MBN) lead to a significant damage of cholinergic neurons concomitant with increased amyloid precursor protein (APP) expression in the cerebral cortex. However, the sensitivity of non-cholinergic neurons to excitotoxicity, and changes of APP expression in the damaged MBN are still elusive. Hence, we performed multiple-labeling immunocytochemistry for choline-acetyltransferase (ChAT), neuron-specific nuclear protein (NeuN) and APP 4, 24, and 48h after NMDA infusion in the MBN. Whereas all cholinergic neurons were immunoreactive for NeuN, this neuronal marker also labeled a population of ChAT-immunonegative non-cholinergic neurons. Both neuron populations exhibited a similar degree of sensitivity to NMDA excitotoxicity that became evident as early as 4 h post-lesion. Cholinergic MBN neurons showed abundant APP immunoreactivity (∼90%), while only a fraction (∼20-30%) of non-cholinergic neurons expressed the protein. Remarkably, cholinergic but not non-cholinergic neurons retained their APP immunoreactivity after NMDA infusion. In conclusion, cholinergic MBN neurons are not preferentially sensitive to short-term excitotoxicity, but are one of the major sources of APP in the basal forebrain.

Original languageEnglish
Pages (from-to)767-772
Number of pages6
JournalNeuroReport
Volume13
Issue number6
Publication statusPublished - May 7 2002

Fingerprint

Amyloid beta-Protein Precursor
Cholinergic Neurons
Neurons
N-Methylaspartate
Choline O-Acetyltransferase
Nuclear Proteins
Amyloidogenic Proteins
Protein Precursors
Cerebral Cortex
Cholinergic Agents
Population
Immunohistochemistry
Proteins

Keywords

  • Basal forebrain
  • Choline-acetyltransferase
  • Confocal laser scanning microscopy
  • Excitotoxicity
  • In vivo labeling
  • Neurodegeneration
  • Neuron-specific nuclear protein
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Harkany, T., Varga, C., Grosche, J., Mulder, J., Luiten, P. G. M., Hortobágyi, T., ... Härtig, W. (2002). Distinct subsets of nucleus basalis neurons exhibit similar sensitivity to excitotoxicity. NeuroReport, 13(6), 767-772.

Distinct subsets of nucleus basalis neurons exhibit similar sensitivity to excitotoxicity. / Harkany, Tibor; Varga, Csaba; Grosche, Jens; Mulder, Jan; Luiten, Paul G M; Hortobágyi, Tibor; Penke, Botond; Härtig, Wolfgang.

In: NeuroReport, Vol. 13, No. 6, 07.05.2002, p. 767-772.

Research output: Contribution to journalArticle

Harkany, T, Varga, C, Grosche, J, Mulder, J, Luiten, PGM, Hortobágyi, T, Penke, B & Härtig, W 2002, 'Distinct subsets of nucleus basalis neurons exhibit similar sensitivity to excitotoxicity', NeuroReport, vol. 13, no. 6, pp. 767-772.
Harkany T, Varga C, Grosche J, Mulder J, Luiten PGM, Hortobágyi T et al. Distinct subsets of nucleus basalis neurons exhibit similar sensitivity to excitotoxicity. NeuroReport. 2002 May 7;13(6):767-772.
Harkany, Tibor ; Varga, Csaba ; Grosche, Jens ; Mulder, Jan ; Luiten, Paul G M ; Hortobágyi, Tibor ; Penke, Botond ; Härtig, Wolfgang. / Distinct subsets of nucleus basalis neurons exhibit similar sensitivity to excitotoxicity. In: NeuroReport. 2002 ; Vol. 13, No. 6. pp. 767-772.
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