Distinct regulation of CAB and PHYB gene expression by similar circadian clocks

Anthony Hall, L. Kozma-Bognár, Ruth M. Bastow, Ferenc Nagy, Andrew J. Millar

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Phytochrome B (phyB) is a major phytochrome active in light-grown plants. The circadian clock controls the expression of the PHYB gene. We have used the luciferase reporter gene (LUC) to monitor the rhythmic expression of PHYB in photoreceptor and clock-associated mutant backgrounds. Surprisingly, we found that PHYB and CAB expression have different free-running periods, indicating that separate circadian clocks control these genes. The effects of mutations show that the clocks share common components. This suggests that they are copies of the same clock mechanism in different locations, most likely in different cell layers. Furthermore, we show that phyB is required for a negative feedback loop that strongly antagonises the expression of PHYB. Compared to a system with only one clock, this regulatory complexity might allow the phase of peak expression for one clock-controlled gene to alter, relative to other genes or to changing environmental conditions.

Original languageEnglish
Pages (from-to)529-537
Number of pages9
JournalPlant Journal
Volume32
Issue number4
DOIs
Publication statusPublished - Nov 2002

Fingerprint

Circadian Clocks
Phytochrome B
phytochrome
circadian rhythm
Gene Expression
gene expression
Phytochrome
Genes
genes
luciferase
Luciferases
photoreceptors
Reporter Genes
reporter genes
mutation
Light
mutants
Mutation
environmental factors
cellulose acetate-butyrate

Keywords

  • Arabidopsis
  • Luciferase
  • Oscillation
  • Phytochrome
  • Transgenic plants

ASJC Scopus subject areas

  • Plant Science

Cite this

Distinct regulation of CAB and PHYB gene expression by similar circadian clocks. / Hall, Anthony; Kozma-Bognár, L.; Bastow, Ruth M.; Nagy, Ferenc; Millar, Andrew J.

In: Plant Journal, Vol. 32, No. 4, 11.2002, p. 529-537.

Research output: Contribution to journalArticle

Hall, Anthony ; Kozma-Bognár, L. ; Bastow, Ruth M. ; Nagy, Ferenc ; Millar, Andrew J. / Distinct regulation of CAB and PHYB gene expression by similar circadian clocks. In: Plant Journal. 2002 ; Vol. 32, No. 4. pp. 529-537.
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