Distinct properties of carbachol- and DHPG-induced network oscillations in hippocampal slices

J. Pálhalmi, O. Paulsen, T. Freund, N. Hájos

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The aim of this study was to compare and contrast the properties of gamma oscillations induced by activation of muscarinic acetylcholine or metabotropic glutamate receptors in the CA3 region of rat hippocampal slices. Both carbachol and the group I metabotropic glutamate receptor agonist, (RS)-3,5- dihydroxyphenylglycine (DHPG), induced network oscillations in the gamma-frequency range (30-100 Hz). The M1 muscarinic receptor antagonist, pirenzepine, blocked carbachol-, but enhanced DHPG-induced oscillations, whereas LY 341495, an antagonist at metabotropic glutamate receptors, abolished DHPG-, but left carbachol-induced oscillations unchanged. There were significant differences in the peak frequency, maximal power, and spectral width of the two oscillations. Pharmacological experiments showed that both types of oscillation depend on fast excitatory and inhibitory synaptic transmission. Interestingly, activation of neurokinin-1 receptors by substance P fragment or enhancement of inhibitory synaptic currents by the benzodiazepine ligand, zolpidem, boosted DHPG-, but reduced the power of carbachol-induced oscillations. These results suggest that, although carbachol and DHPG might activate similar conductances in individual pyramidal cells, the oscillations they induce in slices involve different network mechanisms, most likely by recruiting distinct types of GABAergic interneuron.

Original languageEnglish
Pages (from-to)381-389
Number of pages9
JournalNeuropharmacology
Volume47
Issue number3
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Carbachol
Metabotropic Glutamate Receptors
LY 341495
Hippocampal CA3 Region
Excitatory Amino Acid Agonists
Neurokinin-1 Receptors
Muscarinic Antagonists
Pyramidal Cells
Interneurons
Substance P
Benzodiazepines
Synaptic Transmission
Cholinergic Agents
Acetylcholine
dihydroxyphenylethylene glycol
Pharmacology
Ligands

Keywords

  • Carbachol
  • DHPG
  • Hippocampus
  • Metabotropic glutamate receptor
  • Muscarinic acetylcholine receptor
  • Rat

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Distinct properties of carbachol- and DHPG-induced network oscillations in hippocampal slices. / Pálhalmi, J.; Paulsen, O.; Freund, T.; Hájos, N.

In: Neuropharmacology, Vol. 47, No. 3, 09.2004, p. 381-389.

Research output: Contribution to journalArticle

Pálhalmi, J. ; Paulsen, O. ; Freund, T. ; Hájos, N. / Distinct properties of carbachol- and DHPG-induced network oscillations in hippocampal slices. In: Neuropharmacology. 2004 ; Vol. 47, No. 3. pp. 381-389.
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