Distinct modulation of angiotensin II-induced early left ventricular hypertrophic gene programming by dietary fat type

Gábor Földes, Szilvia Vajda, Zoltán Lakó-Futó, Balázs Sármán, R. Skoumal, Mika Ilves, Rudolf DeChâtel, I. Karádi, Miklós Tóth, Heikki Ruskoaho, I. Leprán

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Long-term dietary fatty acid intake alters the development of left ventricular hypertrophy, but the linking signaling pathways are unclear. We studied the role and underlying signaling mechanisms of dietary fat intake in the early phase of the hypertrophic process. Rats assigned for 4 weeks of high-oil, high-fat, or standard diet were subjected to angiotensin II (Ang II; 33 μg/kg/h, subcutaneous) or vehicle infusion for 24 h. The Ang II-induced increase in left ventricular mRNA levels of hypertrophy-associated genes was higher in rats fed the high-oil diet compared with the standard diet. Western blotting revealed that, in parallel with changes in gene expression, the high-oil diet increased c-Jun N-terminal kinase phosphorylation (P <0.001). Ang II increased p38 mitogen-activated protein kinase (MAPK) phosphorylation in rats fed the high-fat diet (3-fold; P <0.01). The increase in transcription factor activator protein-1 (AP-1) DNA binding activity in response to Ang II was higher in rats fed the high-oil diet compared with those fed the standard diet (P <0.001). Ang II downregulated inducible nitric oxide synthase mRNA levels in fatty acid-supplemented groups compared with the standard diet group. These results show that dietary fat type modulates the early activation of hypertrophic genes in pressure-overloaded myocardium involving the distinct activation of AP-1 and MAPK signal transduction pathways.

Original languageEnglish
Pages (from-to)1219-1226
Number of pages8
JournalJournal of Lipid Research
Volume47
Issue number6
DOIs
Publication statusPublished - Jun 2006

Fingerprint

Dietary Fats
Nutrition
Angiotensin II
Genes
Modulation
Diet
Oils
Rats
Transcription Factor AP-1
Phosphorylation
Fatty Acids
Chemical activation
Fats
Messenger RNA
JNK Mitogen-Activated Protein Kinases
Signal transduction
Left Ventricular Hypertrophy
High Fat Diet
p38 Mitogen-Activated Protein Kinases
Nitric Oxide Synthase Type II

Keywords

  • Cardiac hypertrophy
  • Fatty acids
  • Mitogen-activated protein kinases
  • Signal transduction

ASJC Scopus subject areas

  • Endocrinology

Cite this

Distinct modulation of angiotensin II-induced early left ventricular hypertrophic gene programming by dietary fat type. / Földes, Gábor; Vajda, Szilvia; Lakó-Futó, Zoltán; Sármán, Balázs; Skoumal, R.; Ilves, Mika; DeChâtel, Rudolf; Karádi, I.; Tóth, Miklós; Ruskoaho, Heikki; Leprán, I.

In: Journal of Lipid Research, Vol. 47, No. 6, 06.2006, p. 1219-1226.

Research output: Contribution to journalArticle

Földes, Gábor ; Vajda, Szilvia ; Lakó-Futó, Zoltán ; Sármán, Balázs ; Skoumal, R. ; Ilves, Mika ; DeChâtel, Rudolf ; Karádi, I. ; Tóth, Miklós ; Ruskoaho, Heikki ; Leprán, I. / Distinct modulation of angiotensin II-induced early left ventricular hypertrophic gene programming by dietary fat type. In: Journal of Lipid Research. 2006 ; Vol. 47, No. 6. pp. 1219-1226.
@article{f0965558a36c4f31b6f07f73a5e3efe0,
title = "Distinct modulation of angiotensin II-induced early left ventricular hypertrophic gene programming by dietary fat type",
abstract = "Long-term dietary fatty acid intake alters the development of left ventricular hypertrophy, but the linking signaling pathways are unclear. We studied the role and underlying signaling mechanisms of dietary fat intake in the early phase of the hypertrophic process. Rats assigned for 4 weeks of high-oil, high-fat, or standard diet were subjected to angiotensin II (Ang II; 33 μg/kg/h, subcutaneous) or vehicle infusion for 24 h. The Ang II-induced increase in left ventricular mRNA levels of hypertrophy-associated genes was higher in rats fed the high-oil diet compared with the standard diet. Western blotting revealed that, in parallel with changes in gene expression, the high-oil diet increased c-Jun N-terminal kinase phosphorylation (P <0.001). Ang II increased p38 mitogen-activated protein kinase (MAPK) phosphorylation in rats fed the high-fat diet (3-fold; P <0.01). The increase in transcription factor activator protein-1 (AP-1) DNA binding activity in response to Ang II was higher in rats fed the high-oil diet compared with those fed the standard diet (P <0.001). Ang II downregulated inducible nitric oxide synthase mRNA levels in fatty acid-supplemented groups compared with the standard diet group. These results show that dietary fat type modulates the early activation of hypertrophic genes in pressure-overloaded myocardium involving the distinct activation of AP-1 and MAPK signal transduction pathways.",
keywords = "Cardiac hypertrophy, Fatty acids, Mitogen-activated protein kinases, Signal transduction",
author = "G{\'a}bor F{\"o}ldes and Szilvia Vajda and Zolt{\'a}n Lak{\'o}-Fut{\'o} and Bal{\'a}zs S{\'a}rm{\'a}n and R. Skoumal and Mika Ilves and Rudolf DeCh{\^a}tel and I. Kar{\'a}di and Mikl{\'o}s T{\'o}th and Heikki Ruskoaho and I. Lepr{\'a}n",
year = "2006",
month = "6",
doi = "10.1194/jlr.M500550-JLR200",
language = "English",
volume = "47",
pages = "1219--1226",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "6",

}

TY - JOUR

T1 - Distinct modulation of angiotensin II-induced early left ventricular hypertrophic gene programming by dietary fat type

AU - Földes, Gábor

AU - Vajda, Szilvia

AU - Lakó-Futó, Zoltán

AU - Sármán, Balázs

AU - Skoumal, R.

AU - Ilves, Mika

AU - DeChâtel, Rudolf

AU - Karádi, I.

AU - Tóth, Miklós

AU - Ruskoaho, Heikki

AU - Leprán, I.

PY - 2006/6

Y1 - 2006/6

N2 - Long-term dietary fatty acid intake alters the development of left ventricular hypertrophy, but the linking signaling pathways are unclear. We studied the role and underlying signaling mechanisms of dietary fat intake in the early phase of the hypertrophic process. Rats assigned for 4 weeks of high-oil, high-fat, or standard diet were subjected to angiotensin II (Ang II; 33 μg/kg/h, subcutaneous) or vehicle infusion for 24 h. The Ang II-induced increase in left ventricular mRNA levels of hypertrophy-associated genes was higher in rats fed the high-oil diet compared with the standard diet. Western blotting revealed that, in parallel with changes in gene expression, the high-oil diet increased c-Jun N-terminal kinase phosphorylation (P <0.001). Ang II increased p38 mitogen-activated protein kinase (MAPK) phosphorylation in rats fed the high-fat diet (3-fold; P <0.01). The increase in transcription factor activator protein-1 (AP-1) DNA binding activity in response to Ang II was higher in rats fed the high-oil diet compared with those fed the standard diet (P <0.001). Ang II downregulated inducible nitric oxide synthase mRNA levels in fatty acid-supplemented groups compared with the standard diet group. These results show that dietary fat type modulates the early activation of hypertrophic genes in pressure-overloaded myocardium involving the distinct activation of AP-1 and MAPK signal transduction pathways.

AB - Long-term dietary fatty acid intake alters the development of left ventricular hypertrophy, but the linking signaling pathways are unclear. We studied the role and underlying signaling mechanisms of dietary fat intake in the early phase of the hypertrophic process. Rats assigned for 4 weeks of high-oil, high-fat, or standard diet were subjected to angiotensin II (Ang II; 33 μg/kg/h, subcutaneous) or vehicle infusion for 24 h. The Ang II-induced increase in left ventricular mRNA levels of hypertrophy-associated genes was higher in rats fed the high-oil diet compared with the standard diet. Western blotting revealed that, in parallel with changes in gene expression, the high-oil diet increased c-Jun N-terminal kinase phosphorylation (P <0.001). Ang II increased p38 mitogen-activated protein kinase (MAPK) phosphorylation in rats fed the high-fat diet (3-fold; P <0.01). The increase in transcription factor activator protein-1 (AP-1) DNA binding activity in response to Ang II was higher in rats fed the high-oil diet compared with those fed the standard diet (P <0.001). Ang II downregulated inducible nitric oxide synthase mRNA levels in fatty acid-supplemented groups compared with the standard diet group. These results show that dietary fat type modulates the early activation of hypertrophic genes in pressure-overloaded myocardium involving the distinct activation of AP-1 and MAPK signal transduction pathways.

KW - Cardiac hypertrophy

KW - Fatty acids

KW - Mitogen-activated protein kinases

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=33744796528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744796528&partnerID=8YFLogxK

U2 - 10.1194/jlr.M500550-JLR200

DO - 10.1194/jlr.M500550-JLR200

M3 - Article

C2 - 16569909

AN - SCOPUS:33744796528

VL - 47

SP - 1219

EP - 1226

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 6

ER -