Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis

Enrique López-Juez, Edyta Dillon, Z. Magyar, Safina Khan, Saul Hazeldine, Sarah M. De Jager, James A H Murray, Gerrit T S Beemster, László Bögre, Hugh Shanahan

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

In darkness, shoot apex growth is repressed, but it becomes rapidly activated by light. We show that phytochromes and cryptochromes play largely redundant roles in this derepression in Arabidopsis thaliana. We examined the light activation of transcriptional changes in a finely resolved time course, comparing the shoot apex (meristem and leaf primordia) and the cotyledon and found >5700 differentially expressed genes. Early events specific to the shoot apices included the repression of genes for Really InterestingNewGene finger proteins and basic domain/leucine zipper and basic helix-loop-helix transcription factors. The downregulation of auxin and ethylene and the upregulation of cytokinin and gibberellin hormonal responses were also characteristic of shoot apices. In the apex, genes involved in ribosome biogenesis and protein translation were rapidly and synchronously induced, simultaneously with cell proliferation genes, preceding visible organ growth. Subsequently, the activation of signaling genes and transcriptional signatures of cell wall expansion, turgor generation, and plastid biogenesis were apparent. Furthermore, light regulates the forms and protein levels of two transcription factors with opposing functions in cell proliferation, E2FB and E2FC, through the Constitutively Photomorphogenic1 (COP1), COP9-Signalosome5, and Deetiolated1 light signaling molecules. These data provide the basis for reconstruction of the regulatory networks for light-regulated meristem, leaf, and cotyledon development.

Original languageEnglish
Pages (from-to)947-968
Number of pages22
JournalPlant Cell
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Cotyledon
Arabidopsis
Gene expression
cotyledons
cell cycle
Cell Cycle
Genes
Cells
Gene Expression
Light
gene expression
shoots
Transcriptional Activation
Meristem
Cell proliferation
genes
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
cell proliferation
leaf meristems
Cryptochromes

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

López-Juez, E., Dillon, E., Magyar, Z., Khan, S., Hazeldine, S., De Jager, S. M., ... Shanahan, H. (2008). Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis. Plant Cell, 20(4), 947-968. https://doi.org/10.1105/tpc.107.057075

Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis. / López-Juez, Enrique; Dillon, Edyta; Magyar, Z.; Khan, Safina; Hazeldine, Saul; De Jager, Sarah M.; Murray, James A H; Beemster, Gerrit T S; Bögre, László; Shanahan, Hugh.

In: Plant Cell, Vol. 20, No. 4, 04.2008, p. 947-968.

Research output: Contribution to journalArticle

López-Juez, E, Dillon, E, Magyar, Z, Khan, S, Hazeldine, S, De Jager, SM, Murray, JAH, Beemster, GTS, Bögre, L & Shanahan, H 2008, 'Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis', Plant Cell, vol. 20, no. 4, pp. 947-968. https://doi.org/10.1105/tpc.107.057075
López-Juez, Enrique ; Dillon, Edyta ; Magyar, Z. ; Khan, Safina ; Hazeldine, Saul ; De Jager, Sarah M. ; Murray, James A H ; Beemster, Gerrit T S ; Bögre, László ; Shanahan, Hugh. / Distinct light-initiated gene expression and cell cycle programs in the shoot apex and cotyledons of Arabidopsis. In: Plant Cell. 2008 ; Vol. 20, No. 4. pp. 947-968.
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