Distinct in vitro T-helper 17 differentiation capacity of peripheral naive T cells in rheumatoid and psoriatic arthritis

Eszter Baricza, Nikolett Marton, Panna Királyhidi, Orsolya Tünde Kovács, Ilona Kovácsné Székely, Eszter Lajkó, Lászó Kohidai, Bernadett Rojkovich, Barbara Érsek, E. Búzás, György Nagy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA. Methods: Blood samples from healthy donors, RA and PsA patients were collected. CD45RO- (naive) and CD45RO+ (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulated with anti-CD3, anti-CD28, and goat anti-mouse IgG antibodies and treated with transforming grow factor beta, interleukin (IL)-6, IL-1β, and IL-23 cytokines and also with anti-IL-4 antibody. IL-17A and IL-22 production were measured by enzyme linked immunosorbent assay, RORC, and T-box 21 (TBX21) expression were analyzed by quantitative polymerase chain reaction and flow cytometry. C-C chemokine receptor 6 (CCR6), CCR4, and C-X-C motif chemokine receptor 3 expression were determined by flow cytometry. Cell viability was monitored by impedance-based cell analyzer (CASY-TT). Results: RORC, TBX21, CCR6, and CCR4 expression of memory T cells of healthy individuals (but not RA or PsA patients) were increased (p < 0.01; p < 0.001; p < 0.05; p < 0.05, respectively) compared to the naive cells. Cytokine-induced IL-17A production was different in both RA and PsA patients when compared to healthy donors (p = 0.0000026 and p = 0.0001047, respectively). By contrast, significant differences in IL-22 production were observed only between RA versus healthy or RA versus PsA patients (p = 0.000006; p = 0.0013454, respectively), but not between healthy donors versus PsA patients. Conclusion: The naive CD4 T-lymphocytes are predisposed to differentiate into Th17 cells and the in vitro Th17 cell differentiation is profoundly altered in both RA and PsA.

Original languageEnglish
Article number606
JournalFrontiers in Immunology
Volume9
Issue numberAPR
DOIs
Publication statusPublished - Apr 4 2018

Fingerprint

Psoriatic Arthritis
Th17 Cells
Rheumatoid Arthritis
Chemokine Receptors
T-Lymphocytes
CC Chemokines
Interleukin-17
Tissue Donors
Cell Differentiation
Flow Cytometry
Cytokines
Interleukin-23
CXC Chemokines
Antibodies
Cell Separation
Electric Impedance
Interleukin-1
Goats
Interleukin-4
Interleukin-6

Keywords

  • Interleukin-17A
  • Interleukin-22
  • Psoriatic arthritis
  • Rheumatoid arthritis
  • T-helper 17 differentiation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Baricza, E., Marton, N., Királyhidi, P., Kovács, O. T., Székely, I. K., Lajkó, E., ... Nagy, G. (2018). Distinct in vitro T-helper 17 differentiation capacity of peripheral naive T cells in rheumatoid and psoriatic arthritis. Frontiers in Immunology, 9(APR), [606]. https://doi.org/10.3389/fimmu.2018.00606

Distinct in vitro T-helper 17 differentiation capacity of peripheral naive T cells in rheumatoid and psoriatic arthritis. / Baricza, Eszter; Marton, Nikolett; Királyhidi, Panna; Kovács, Orsolya Tünde; Székely, Ilona Kovácsné; Lajkó, Eszter; Kohidai, Lászó; Rojkovich, Bernadett; Érsek, Barbara; Búzás, E.; Nagy, György.

In: Frontiers in Immunology, Vol. 9, No. APR, 606, 04.04.2018.

Research output: Contribution to journalArticle

Baricza, E, Marton, N, Királyhidi, P, Kovács, OT, Székely, IK, Lajkó, E, Kohidai, L, Rojkovich, B, Érsek, B, Búzás, E & Nagy, G 2018, 'Distinct in vitro T-helper 17 differentiation capacity of peripheral naive T cells in rheumatoid and psoriatic arthritis', Frontiers in Immunology, vol. 9, no. APR, 606. https://doi.org/10.3389/fimmu.2018.00606
Baricza, Eszter ; Marton, Nikolett ; Királyhidi, Panna ; Kovács, Orsolya Tünde ; Székely, Ilona Kovácsné ; Lajkó, Eszter ; Kohidai, Lászó ; Rojkovich, Bernadett ; Érsek, Barbara ; Búzás, E. ; Nagy, György. / Distinct in vitro T-helper 17 differentiation capacity of peripheral naive T cells in rheumatoid and psoriatic arthritis. In: Frontiers in Immunology. 2018 ; Vol. 9, No. APR.
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abstract = "Background: The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA. Methods: Blood samples from healthy donors, RA and PsA patients were collected. CD45RO- (naive) and CD45RO+ (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulated with anti-CD3, anti-CD28, and goat anti-mouse IgG antibodies and treated with transforming grow factor beta, interleukin (IL)-6, IL-1β, and IL-23 cytokines and also with anti-IL-4 antibody. IL-17A and IL-22 production were measured by enzyme linked immunosorbent assay, RORC, and T-box 21 (TBX21) expression were analyzed by quantitative polymerase chain reaction and flow cytometry. C-C chemokine receptor 6 (CCR6), CCR4, and C-X-C motif chemokine receptor 3 expression were determined by flow cytometry. Cell viability was monitored by impedance-based cell analyzer (CASY-TT). Results: RORC, TBX21, CCR6, and CCR4 expression of memory T cells of healthy individuals (but not RA or PsA patients) were increased (p < 0.01; p < 0.001; p < 0.05; p < 0.05, respectively) compared to the naive cells. Cytokine-induced IL-17A production was different in both RA and PsA patients when compared to healthy donors (p = 0.0000026 and p = 0.0001047, respectively). By contrast, significant differences in IL-22 production were observed only between RA versus healthy or RA versus PsA patients (p = 0.000006; p = 0.0013454, respectively), but not between healthy donors versus PsA patients. Conclusion: The naive CD4 T-lymphocytes are predisposed to differentiate into Th17 cells and the in vitro Th17 cell differentiation is profoundly altered in both RA and PsA.",
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AU - Baricza, Eszter

AU - Marton, Nikolett

AU - Királyhidi, Panna

AU - Kovács, Orsolya Tünde

AU - Székely, Ilona Kovácsné

AU - Lajkó, Eszter

AU - Kohidai, Lászó

AU - Rojkovich, Bernadett

AU - Érsek, Barbara

AU - Búzás, E.

AU - Nagy, György

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N2 - Background: The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA. Methods: Blood samples from healthy donors, RA and PsA patients were collected. CD45RO- (naive) and CD45RO+ (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulated with anti-CD3, anti-CD28, and goat anti-mouse IgG antibodies and treated with transforming grow factor beta, interleukin (IL)-6, IL-1β, and IL-23 cytokines and also with anti-IL-4 antibody. IL-17A and IL-22 production were measured by enzyme linked immunosorbent assay, RORC, and T-box 21 (TBX21) expression were analyzed by quantitative polymerase chain reaction and flow cytometry. C-C chemokine receptor 6 (CCR6), CCR4, and C-X-C motif chemokine receptor 3 expression were determined by flow cytometry. Cell viability was monitored by impedance-based cell analyzer (CASY-TT). Results: RORC, TBX21, CCR6, and CCR4 expression of memory T cells of healthy individuals (but not RA or PsA patients) were increased (p < 0.01; p < 0.001; p < 0.05; p < 0.05, respectively) compared to the naive cells. Cytokine-induced IL-17A production was different in both RA and PsA patients when compared to healthy donors (p = 0.0000026 and p = 0.0001047, respectively). By contrast, significant differences in IL-22 production were observed only between RA versus healthy or RA versus PsA patients (p = 0.000006; p = 0.0013454, respectively), but not between healthy donors versus PsA patients. Conclusion: The naive CD4 T-lymphocytes are predisposed to differentiate into Th17 cells and the in vitro Th17 cell differentiation is profoundly altered in both RA and PsA.

AB - Background: The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA. Methods: Blood samples from healthy donors, RA and PsA patients were collected. CD45RO- (naive) and CD45RO+ (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulated with anti-CD3, anti-CD28, and goat anti-mouse IgG antibodies and treated with transforming grow factor beta, interleukin (IL)-6, IL-1β, and IL-23 cytokines and also with anti-IL-4 antibody. IL-17A and IL-22 production were measured by enzyme linked immunosorbent assay, RORC, and T-box 21 (TBX21) expression were analyzed by quantitative polymerase chain reaction and flow cytometry. C-C chemokine receptor 6 (CCR6), CCR4, and C-X-C motif chemokine receptor 3 expression were determined by flow cytometry. Cell viability was monitored by impedance-based cell analyzer (CASY-TT). Results: RORC, TBX21, CCR6, and CCR4 expression of memory T cells of healthy individuals (but not RA or PsA patients) were increased (p < 0.01; p < 0.001; p < 0.05; p < 0.05, respectively) compared to the naive cells. Cytokine-induced IL-17A production was different in both RA and PsA patients when compared to healthy donors (p = 0.0000026 and p = 0.0001047, respectively). By contrast, significant differences in IL-22 production were observed only between RA versus healthy or RA versus PsA patients (p = 0.000006; p = 0.0013454, respectively), but not between healthy donors versus PsA patients. Conclusion: The naive CD4 T-lymphocytes are predisposed to differentiate into Th17 cells and the in vitro Th17 cell differentiation is profoundly altered in both RA and PsA.

KW - Interleukin-17A

KW - Interleukin-22

KW - Psoriatic arthritis

KW - Rheumatoid arthritis

KW - T-helper 17 differentiation

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