Distinct claudin expression profile in histologic subtypes of lung cancer

Judit Moldvay, Márta Jäckel, C. Páska, Ibolya Soltész, Z. Schaff, András Kiss

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Purpose: Claudins are the main constituents of tight junctions. Little is known about their expression and localization in the normal bronchial epithelium and in lung cancer. Patients and methods: One hundred four lung cancer tissue blocks were studied including 46 adenocarcinomas (ADC), 30 squamous cell carcinomas (SCC), 15 small cell lung cancers (SCLC), 8 typical and 5 atypical carcinoids. All slides contained normal bronchial mucosa as well. Immunohistochemistry using antibodies against claudins-1, -2, -3, -4, and -7 proteins, as well as semi-quantitative estimation were performed. RT-PCR analysis was also carried out in 22 immunohistochemically representative tumor samples. Results: Normal bronchial epithelial cells expressed all the examined claudin proteins. When compared, SCLCs and carcinoids showed striking differences in regard to claudins-1, -3, and -4 expressions (p <0.0001, p <0.0001, and p <0.0004, respectively), whereas ADCs and SCCs revealed significant differences in claudins-3, -4, and -7 expressions (p <0.0001, p <0.0001, and p <0.0053, respectively). However, comparison of ADCs with SCLCs revealed significant difference only in claudin-2 expression (p <0.0002). The comparison of ADCs and carcinoids resulted in significant differences regarding claudins-1, -3, and -4 expressions (p <0.0006, p <0.0001, and p <0.0001, respectively). SCCs and SCLCs varied in respect to claudin-2, -3, and -4 expressions (p <0.0009, p <0.0001, and p <0.0019, respectively), whereas SCCs and carcinoids showed different claudins-1 and -4 expressions (p <0.0076 and p <0.0045, respectively). RT-PCR analysis revealed parallel changes in the mRNA and protein expression of certain claudins. Conclusions: The observed distinct claudin expression profile within the non-small cell lung cancer group, further, the marked differences between SCLCs and carcinoids may have differential diagnostic impact, and the overexpression of certain claudins might have therapeutic implications.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalLung Cancer
Volume57
Issue number2
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Claudins
Lung Neoplasms
Carcinoid Tumor
Claudin-2
Claudin-3
Polymerase Chain Reaction
Tight Junctions
Small Cell Lung Carcinoma
Non-Small Cell Lung Carcinoma
Squamous Cell Carcinoma
Mucous Membrane
Proteins
Adenocarcinoma
Epithelium
Epithelial Cells
Immunohistochemistry

Keywords

  • Bronchial epithelium
  • Claudin
  • Differential diagnosis
  • Immunohistochemistry
  • Lung cancer
  • RT-PCR
  • Tight junction proteins

ASJC Scopus subject areas

  • Oncology

Cite this

Distinct claudin expression profile in histologic subtypes of lung cancer. / Moldvay, Judit; Jäckel, Márta; Páska, C.; Soltész, Ibolya; Schaff, Z.; Kiss, András.

In: Lung Cancer, Vol. 57, No. 2, 08.2007, p. 159-167.

Research output: Contribution to journalArticle

Moldvay, Judit ; Jäckel, Márta ; Páska, C. ; Soltész, Ibolya ; Schaff, Z. ; Kiss, András. / Distinct claudin expression profile in histologic subtypes of lung cancer. In: Lung Cancer. 2007 ; Vol. 57, No. 2. pp. 159-167.
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AU - Moldvay, Judit

AU - Jäckel, Márta

AU - Páska, C.

AU - Soltész, Ibolya

AU - Schaff, Z.

AU - Kiss, András

PY - 2007/8

Y1 - 2007/8

N2 - Purpose: Claudins are the main constituents of tight junctions. Little is known about their expression and localization in the normal bronchial epithelium and in lung cancer. Patients and methods: One hundred four lung cancer tissue blocks were studied including 46 adenocarcinomas (ADC), 30 squamous cell carcinomas (SCC), 15 small cell lung cancers (SCLC), 8 typical and 5 atypical carcinoids. All slides contained normal bronchial mucosa as well. Immunohistochemistry using antibodies against claudins-1, -2, -3, -4, and -7 proteins, as well as semi-quantitative estimation were performed. RT-PCR analysis was also carried out in 22 immunohistochemically representative tumor samples. Results: Normal bronchial epithelial cells expressed all the examined claudin proteins. When compared, SCLCs and carcinoids showed striking differences in regard to claudins-1, -3, and -4 expressions (p <0.0001, p <0.0001, and p <0.0004, respectively), whereas ADCs and SCCs revealed significant differences in claudins-3, -4, and -7 expressions (p <0.0001, p <0.0001, and p <0.0053, respectively). However, comparison of ADCs with SCLCs revealed significant difference only in claudin-2 expression (p <0.0002). The comparison of ADCs and carcinoids resulted in significant differences regarding claudins-1, -3, and -4 expressions (p <0.0006, p <0.0001, and p <0.0001, respectively). SCCs and SCLCs varied in respect to claudin-2, -3, and -4 expressions (p <0.0009, p <0.0001, and p <0.0019, respectively), whereas SCCs and carcinoids showed different claudins-1 and -4 expressions (p <0.0076 and p <0.0045, respectively). RT-PCR analysis revealed parallel changes in the mRNA and protein expression of certain claudins. Conclusions: The observed distinct claudin expression profile within the non-small cell lung cancer group, further, the marked differences between SCLCs and carcinoids may have differential diagnostic impact, and the overexpression of certain claudins might have therapeutic implications.

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