Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90

Z. Lele, Steven D. Hartson, C. Cristofre Martin, Luke Whitesell, Robert L. Matts, Patrick H. Krone

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Members of the Hsp90 family of molecular chaperones play important roles in allowing some intracellular signaling molecules and transcription factors to reach and maintain functionally active conformations. In the present study, we have utilized the specific Hsp90-binding agent, geldanamycin, to examine the requirement for Hsp90 during zebrafish development. We show that geldanamycin interacts with both the α and the β-isoforms of zebrafish Hsp90 and that geldanamycin-treated embryos consistently exhibit a number of defects in tissues which express either one of these genes. Within the somites, geldanamycin treatment results in the absence of eng-2-expressing muscle pioneer cells. However, early development of adaxial cells, which give rise to muscle pioneers and which strongly express the hsp90α gene shortly before muscle pioneer formation, appeared unaffected. Furthermore, development of the notochord, which provides many of the signals required for proper somite patterning and which does not express detectable levels of either hsp90α or hsp90β mRNA, was similarly unaffected in geldanamycin- treated embryos. The data are consistent with there being a temporal and spatial requirement for Hsp90 function within somitic cells which is necessary for the formation of eng-2-expressing muscle pioneers and possibly other striated muscle fiber types.

Original languageEnglish
Pages (from-to)56-70
Number of pages15
JournalDevelopmental Biology
Volume210
Issue number1
DOIs
Publication statusPublished - Jun 1 1999

Fingerprint

Somites
Zebrafish
Muscles
Embryonic Structures
Notochord
Molecular Chaperones
Striated Muscle
Muscle Cells
Genes
Protein Isoforms
Transcription Factors
geldanamycin
Inhibition (Psychology)
Messenger RNA

Keywords

  • Geldanamycin
  • Hsp90
  • Muscle development
  • MyoD
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Lele, Z., Hartson, S. D., Martin, C. C., Whitesell, L., Matts, R. L., & Krone, P. H. (1999). Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90. Developmental Biology, 210(1), 56-70. https://doi.org/10.1006/dbio.1999.9262

Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90. / Lele, Z.; Hartson, Steven D.; Martin, C. Cristofre; Whitesell, Luke; Matts, Robert L.; Krone, Patrick H.

In: Developmental Biology, Vol. 210, No. 1, 01.06.1999, p. 56-70.

Research output: Contribution to journalArticle

Lele, Z, Hartson, SD, Martin, CC, Whitesell, L, Matts, RL & Krone, PH 1999, 'Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90', Developmental Biology, vol. 210, no. 1, pp. 56-70. https://doi.org/10.1006/dbio.1999.9262
Lele, Z. ; Hartson, Steven D. ; Martin, C. Cristofre ; Whitesell, Luke ; Matts, Robert L. ; Krone, Patrick H. / Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90. In: Developmental Biology. 1999 ; Vol. 210, No. 1. pp. 56-70.
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