Displacement and spacer displacement chromatography. Alteration of spot profile in displacement thin-layer chromatography

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4 Citations (Scopus)

Abstract

Migration of spots in displacement development was investigated using a planar stationary phase. The effects of the presence or absence of the additive substances in the carrier were demonstrated when these compounds also played a role in the competition for the binding sites of the stationary phase; however, they were not members of the displacement train. In the case of displacement chromatography, compounds A+B are well separated but located very closely to each other. Using a spacer (C) in the sample, the peaks or spots of A and B are aparted by C and their virtual separation is increased. When compounds A+B were displaced by each other as well as by the displacer, the presence of the additive compound C in the carrier (carrier-spacer) can transfer an otherwise displaced component (A) into the so-called overloaded elution region of the chromatogram. At the same time the additive compound C does not unconditionally reduce the performance of displacement chromatography.

Original languageEnglish
Pages (from-to)253-256
Number of pages4
JournalChromatographia
Volume27
Issue number5-6
DOIs
Publication statusPublished - Mar 1 1989

Keywords

  • Displacement TLC
  • Displacement chromatography
  • Overloaded elution chromatography
  • Spacer displacement thin-layer chromatography
  • Spot profile

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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